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Visualization of tegument-capsid interactions and DNA in intact herpes simplex virus type 1 virions

Zhou, Z.H., Chen, D.H., Jakana, J., Rixon, F.J., and Chiu, W. (1999) Visualization of tegument-capsid interactions and DNA in intact herpes simplex virus type 1 virions. Journal of Virology, 73 (4). pp. 3210-3218. ISSN 0022-538X

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Publisher's URL: http://jvi.asm.org/content/73/4/3210.long

Abstract

Herpes simplex virus type 1 virions were examined by electron cryomicroscopy, allowing the three-dimensional structure of the infectious particle to be visualized for the first time. The capsid shell is identical to that of B-capsids purified from the host cell nucleus, with the exception of the penton channel, which is closed. The double-stranded DNA genome is organized as regularly spaced (approximately 26 A) concentric layers inside the capsid. This pattern suggests a spool model for DNA packaging, similar to that for some bacteriophages. The bulk of the tegument is not icosahedrally ordered. However, a small portion appears as filamentous structures around the pentons, interacting extensively with the capsid. Their locations and interactions suggest possible roles for the tegument proteins in regulating DNA transport through the penton channel and binding to cellular transport proteins during viral infection.

Item Type:Article
Status:Published
Refereed:Yes
Glasgow Author(s):Rixon, Dr Frazer
Authors: Zhou, Z.H., Chen, D.H., Jakana, J., Rixon, F.J., and Chiu, W.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation > Centre for Virus Research
Journal Name:Journal of Virology
Journal Abbr.:J. Virol.
ISSN:0022-538X
ISSN (Online):1098-5514

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