Zhou, Z.H., Chiu, W., Haskell, K., Spears, H., Jakana, J., Rixon, F.J., and Scott, L.R. (1998) Refinement of herpesvirus B-capsid structure on parallel supercomputers. Biophysical Journal, 74 (1). pp. 576-588. ISSN 0006-3495 (doi:10.1016/S0006-3495(98)77816-6)
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Publisher's URL: http://dx.doi.org/10.1016/S0006-3495(98)77816-6
Electron cryomicroscopy and icosahedral reconstruction are used to obtain the three-dimensional structure of the 1250-A-diameter herpesvirus B-capsid. The centers and orientations of particles in focal pairs of 400-kV, spot-scan micrographs are determined and iteratively refined by common-lines-based local and global refinement procedures. We describe the rationale behind choosing shared-memory multiprocessor computers for executing the global refinement, which is the most computationally intensive step in the reconstruction procedure. This refinement has been implemented on three different shared-memory supercomputers. The speedup and efficiency are evaluated by using test data sets with different numbers of particles and processors. Using this parallel refinement program, we refine the herpesvirus B-capsid from 355-particle images to 13-A resolution. The map shows new structural features and interactions of the protein subunits in the three distinct morphological units: penton, hexon, and triplex of this T = 16 icosahedral particle.
|Glasgow Author(s) Enlighten ID:||Rixon, Dr Frazer|
|Authors:||Zhou, Z.H., Chiu, W., Haskell, K., Spears, H., Jakana, J., Rixon, F.J., and Scott, L.R.|
|College/School:||College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation > Centre for Virus Research|
|Research Group:||Biophys. J.|
|Journal Name:||Biophysical Journal|