Zhou, Z.H., Macnab, S., Jakana, J., Scott, L.R., Chiu, W., and Rixon, F.J. (1998) Identification of the sites of interaction between the scaffold and outer shell in herpes simplex virus-1 capsids by difference electron imaging. Proceedings of the National Academy of Sciences of the United States of America, 95 (6). pp. 2778-2783. ISSN 0027-8424 (doi:10.1073/pnas.95.6.2778)
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Publisher's URL: http://dx.doi.org/10.1073/pnas.95.6.2778
Formation of herpes simplex virus-1 capsids requires the presence of intact scaffolding proteins. The C terminus of the abundant scaffolding protein associates with the major capsid shell protein VP5 through hydrophobic interactions. After cleavage by the viral encoded protease, which removes their C-terminal 25 aa, the scaffolding proteins are released from the capsid. We have used electron cryomicroscopy and computer image processing to determine, to 13 Å, the three-dimensional structures of capsids containing either cleaved or uncleaved scaffolding proteins. Detailed comparisons show that the structures of the outer icosahedral shells are almost identical in the two capsid types. Differences are apparent in the radial distribution of the density inside the capsid shell (within a radius of 460 Å) which represents the scaffolding core. However, in both capsid types, the bulk of this internal density exhibits no icosahedral symmetry. Close examination revealed localized regions of icosahedrally arranged extra density at the interface between the outer shell and the scaffold of protease-minus capsids. Rod-like densities extending inwards for ≈40 Å from the capsid shell are present under four of the six quasi-equivalent triplex positions. Under triplexes Tb, Tc, and Te, the major additional densities appear as pairs with the rods in each pair situated 37 Å apart. We propose that these rods are formed by the C-termini of the scaffolding proteins and represent the sites of interaction between the capsid shell and scaffold.
|Glasgow Author(s):||Rixon, Dr Frazer|
|Authors:||Zhou, Z.H., Macnab, S., Jakana, J., Scott, L.R., Chiu, W., and Rixon, F.J.|
|College/School:||College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation > Centre for Virus Research|
|Journal Name:||Proceedings of the National Academy of Sciences of the United States of America|