Positive impact of pre-stroke surgery on survival following transient focal ischemia in hypertensive rats

Ord, E. N.J. , Shirley, R., van Kralingen, J. C., Graves, A., McClure, J. D. , Wilkinson, M., McCabe, C. , Macrae, I. M. and Work, L. M. (2012) Positive impact of pre-stroke surgery on survival following transient focal ischemia in hypertensive rats. Journal of Neuroscience Methods, 211(2), pp. 305-308. (doi:10.1016/j.jneumeth.2012.09.001)

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Abstract

We describe a positive influence of pre-stroke surgery on recovery and survival in a commonly used experimental stroke model. Two groups of male, stroke-prone spontaneously hypertensive rats (SHRSPs) underwent transient middle cerebral artery occlusion (tMCAO). Group 1 underwent the procedure without any prior intervention whilst group 2 had an additional general anaesthetic 6 days prior to tMCAO for a cranial burrhole and durotomy. Post-stroke recovery was assessed using a 32 point neurological deficit score and tapered beam walk and infarct volume determined from haematoxylin–eosin stained sections. In group 2 survival was 92% (n = 12) versus 67% in group 1 (n = 18). In addition, post-tMCAO associated weight loss was significantly reduced in group 2. There was no significant difference between the two groups in experimental outcomes: infarct volume (Group 1 317 ± 18.6 mm<sup>3</sup> versus Group 2 332 ± 20.4 mm<sup>3</sup>), and serial (day 0–14 post-tMCAO) neurological deficit scores and tapered-beam walk test. Drilling a cranial burrhole under general anaesthesia prior to tMCAO in SHRSP reduced mortality and gave rise to infarct volumes and neurological deficits similar to those recorded in surviving Group 1 animals. This methodological refinement has significant implications for animal welfare and group sizes required for intervention studies.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Shirley, Dr Rachel and Macrae, Professor I Mhairi and Ord, Dr Emily and McCabe, Dr Chris and Wilkinson, Dr Michael and Work, Dr Lorraine and Van Kralingen, Ms Josie and McClure, Dr John
Authors: Ord, E. N.J., Shirley, R., van Kralingen, J. C., Graves, A., McClure, J. D., Wilkinson, M., McCabe, C., Macrae, I. M., and Work, L. M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
College of Medical Veterinary and Life Sciences > Institute of Neuroscience and Psychology
Journal Name:Journal of Neuroscience Methods
Publisher:Elsevier BV
ISSN:0165-0270
ISSN (Online):1872-678X
Published Online:10 September 2012
Copyright Holders:Copyright © 2012 The Authors
First Published:First published in Journal of Neuroscience Methods 211(2):305-308
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
469451A combined approach targeting oxidative stress and apoptosis in strokeLorraine WorkBritish Heart Foundation (BHF)PG/07/126/24223RI CARDIOVASCULAR & MEDICAL SCIENCES