Positive impact of pre-stroke surgery on survival following transient focal ischemia in hypertensive rats

Ord, E. N.J. , Shirley, R., van Kralingen, J. C., Graves, A., McClure, J. D. , Wilkinson, M., McCabe, C. , Macrae, I. M. and Work, L. M. (2012) Positive impact of pre-stroke surgery on survival following transient focal ischemia in hypertensive rats. Journal of Neuroscience Methods, 211(2), pp. 305-308. (doi: 10.1016/j.jneumeth.2012.09.001)

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Abstract

We describe a positive influence of pre-stroke surgery on recovery and survival in a commonly used experimental stroke model. Two groups of male, stroke-prone spontaneously hypertensive rats (SHRSPs) underwent transient middle cerebral artery occlusion (tMCAO). Group 1 underwent the procedure without any prior intervention whilst group 2 had an additional general anaesthetic 6 days prior to tMCAO for a cranial burrhole and durotomy. Post-stroke recovery was assessed using a 32 point neurological deficit score and tapered beam walk and infarct volume determined from haematoxylin–eosin stained sections. In group 2 survival was 92% (n = 12) versus 67% in group 1 (n = 18). In addition, post-tMCAO associated weight loss was significantly reduced in group 2. There was no significant difference between the two groups in experimental outcomes: infarct volume (Group 1 317 ± 18.6 mm<sup>3</sup> versus Group 2 332 ± 20.4 mm<sup>3</sup>), and serial (day 0–14 post-tMCAO) neurological deficit scores and tapered-beam walk test. Drilling a cranial burrhole under general anaesthesia prior to tMCAO in SHRSP reduced mortality and gave rise to infarct volumes and neurological deficits similar to those recorded in surviving Group 1 animals. This methodological refinement has significant implications for animal welfare and group sizes required for intervention studies.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Macrae, Professor Mhairi and Ord, Dr Emily and Wilkinson, Dr Michael and Van Kralingen, Ms Josie and Masson, Dr Rachel and McCabe, Dr Chris and Work, Dr Lorraine and McClure, Dr John
Authors: Ord, E. N.J., Shirley, R., van Kralingen, J. C., Graves, A., McClure, J. D., Wilkinson, M., McCabe, C., Macrae, I. M., and Work, L. M.
College/School:College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
College of Medical Veterinary and Life Sciences > School of Psychology & Neuroscience
Journal Name:Journal of Neuroscience Methods
Publisher:Elsevier BV
ISSN:0165-0270
ISSN (Online):1872-678X
Published Online:10 September 2012
Copyright Holders:Copyright © 2012 The Authors
First Published:First published in Journal of Neuroscience Methods 211(2):305-308
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
469451A combined approach targeting oxidative stress and apoptosis in strokeLorraine WorkBritish Heart Foundation (BHF)PG/07/126/24223RI CARDIOVASCULAR & MEDICAL SCIENCES