The products of gene US11 of Herpes Simplex Virus type 1 are DNA-binding and localize to the nucleoli of infected cells

Abstract

We have used antisera raised against synthetic oligopeptides to characterize the protein products from herpes simplex virus type 1 gene Usll. These antisera recognized predominantly polypeptides of apparent molecular weight 21000 and 22000, but also polypeptides of apparent molecular weight 17500, 15000, 14000 and 1 1000. Tryptic peptide fingerprint analysis confirmed that these polypeptides were all closely related. The 21000 and 22 000 molecular weight polypeptides were shown to be DNA-binding proteins, and immune electron microscopy demonstrated their strong localization within nucleoli of infected cells.