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Reactivation in vivo and in vitro of herpes simplex virus from mouse dorsal root ganglia which contain different levels of latency-associated transcripts

Ecob-Prince, M.S., Rixon, F.J., Preston, C.M., Hassan, K., and Kennedy, P.G.E. (1993) Reactivation in vivo and in vitro of herpes simplex virus from mouse dorsal root ganglia which contain different levels of latency-associated transcripts. Journal of General Virology, 74 (6). pp. 995-1002. ISSN 0022-1317 (doi:10.1099/0022-1317-74-6-995)

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Publisher's URL: http://dx.doi.org/10.1099/0022-1317-74-6-995

Abstract

In the dorsal root ganglia (DRG) of mice latently infected with the herpes simplex virus type 1 mutant in1814, there are more neurons that contain latencyassociated transcripts (LATs) than in DRG of mice infected with a dose of equal infectivity of either a revertant or a wild-type virus. We investigated whether higher levels ofLAT + neurons resulted in more extensive reactivation either in vivo following neurectomy of the sciatic nerve or in vitro after explantation into culture. Neurectomy appeared to induce expression of immediate early 1 mRNA (IElmRNA) in neurons of mice latently infected with each of three viruses. However IElmRNA was detected in no more than 0"25 % of the neurons of DRG from animals 2 to 4 days after neurectomy, irrespective of the percentage of LAT ÷ neurons present. Of the 22 neurons shown to express IElmRNA, none expressed LATs also. However the lack of expression of viral antigen and the absence of a reduced potential for reactivation on explantation suggested that neurectomy had not induced full reactivation involving lytic replication leading to the death of the latently infected neurons. When DRG were explanted into culture, the distribution of the frequency of reactivation was similar to the distribution of DRG that contained LAT + neurons. The presence of a high proportion of LAT + neurons was not directly associated with earlier detection of reactivation but such experiments cannot be regarded as quantitative. We therefore concluded that neurectomy did not result in a reduced reactivation potential as described by others and that the frequency of expression of IElmRNA following neurectomy did not correlate with the number of LAT + nuerons present.

Item Type:Article
Status:Published
Refereed:Yes
Glasgow Author(s):Rixon, Dr Frazer and Preston, Prof Christopher
Authors: Ecob-Prince, M.S., Rixon, F.J., Preston, C.M., Hassan, K., and Kennedy, P.G.E.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of General Virology
Journal Abbr.:J. Gen. Virol.
Publisher:Society for General Microbiology
ISSN:0022-1317
ISSN (Online):1465-2099

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