Enlighten
Research publications by members of the University of Glasgow
home > services > Enlighten

Characterisation of a herpes simplex virus type 1 mutant which has a temperature-sensitive defect in penetration of cells and assembly of capsids

Addison, C., Rixon, F.J., Palfreyman, J.W., O'Hara, M., and Preston, V.G. (1984) Characterisation of a herpes simplex virus type 1 mutant which has a temperature-sensitive defect in penetration of cells and assembly of capsids. Virology, 138 (2). pp. 246-259. ISSN 0042-6822 (doi:10.1016/0042-6822(84)90349-0)

Full text not currently available from Enlighten.

Abstract

A herpes simplex virus type 1 (HSV-1) mutant, ts1204, which has a temperature-sensitive (ts) mutation located within genome map coordinates 0.318 to 0.324, close to but outside the coding sequences of the glycoprotein gB gene, has been characterised. Although this mutant adsorbed to the cell surface at the nonpermissive temperature (NPT), it failed to penetrate the cell membrane. As a consequence of this defect, high multiplicities of infection of ts1204 blocked subsequent infection of cells by wild-type HSV-1. By contrast, at the NPT, superinfection of cells with HSV-2 was not inhibited by prior infection with ts1204. The penetration defect could be overcome either by brief incubation of mutant virus-infected cells at the permissive temperature, or by treatment of the cells with polyethylene glycol, a compound which promotes fusion of membranes. Upon continued incubation of ts1204-infected cells at the NPT, low numbers of capsids were assembled. Although these capsids all had some internal structure, they did not contain DNA. Another mutant, ts1208, which lies in the same complementation group as ts1204, penetrated cells normally at the NPT, but like ts1204, had a defect in the formation of functional capsids. Evidence presented in this paper suggests that the gene in which the ts1204 and ts1208 lesions map encodes a structural polypeptide.

Item Type:Article
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:O'Hara, Mrs Maureen and Rixon, Dr Frazer and Preston, Dr Valerie
Authors: Addison, C., Rixon, F.J., Palfreyman, J.W., O'Hara, M., and Preston, V.G.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Virology
ISSN:0042-6822

University Staff: Request a correction | Enlighten Editors: Update this record