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Inducible degradation of Ikappa Balpha by the proteasome requires interaction with the F-box protein h-beta TrCP

Kroll, M., Margottin, F., Kohl, A., Renard, P., Durand, H., Concordet, J., Bachelerie, F., Arenzana-Seisdedos, F., and Benarous, R. (1999) Inducible degradation of Ikappa Balpha by the proteasome requires interaction with the F-box protein h-beta TrCP. Journal of Biological Chemistry, 274 (12). pp. 7941-7945. ISSN 0021-9258 (doi:10.1074/jbc.274.12.7941)

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Publisher's URL: http://dx.doi.org/10.1074/jbc.274.12.7941

Abstract

Activation of NF-kappaB transcription factors requires phosphorylation and ubiquitin-proteasome-dependent degradation of IkappaB proteins. We provide evidence that a human F-box protein, h-betaTrCP, a component of Skp1-Cullin-F-box protein (SCF) complexes, a new class of E3 ubiquitin ligases, is essential for inducible degradation of IkappaBalpha. betaTrCP associates with Ser32-Ser36 phosphorylated, but not with unmodified IkappaBalpha or Ser32-Ser36 phosphorylation-deficient mutants. Expression of a F-box-deleted betaTrCP inhibits IkappaBalpha degradation, promotes accumulation of phosphorylated Ser32-Ser36 IkappaBalpha, and prevents NF-kappaB-dependent transcription. Our findings indicate that betaTrCP is the adaptor protein required for IkappaBalpha recognition by the SCFbetaTrCP E3 complex that ubiquitinates IkappaBalpha and makes it a substrate for the proteasome.

Item Type:Article
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Kohl, Dr Alain
Authors: Kroll, M., Margottin, F., Kohl, A., Renard, P., Durand, H., Concordet, J., Bachelerie, F., Arenzana-Seisdedos, F., and Benarous, R.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation > Centre for Virus Research
Journal Name:Journal of Biological Chemistry
Journal Abbr.:J. Biol. Chem
Publisher:American Society for Biochemistry and Molecular Biology, Inc.
ISSN:0021-9258
ISSN (Online):1083-351X

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