The development of a ε-polycaprolactone (PCL) scaffold for CNS repair

Donoghue, P.S., Lamond, R., Boomkamp, S.D., Tao, S., Gadegaard, N. , Riehle, M.O. and Barnett, S.C. (2013) The development of a ε-polycaprolactone (PCL) scaffold for CNS repair. Tissue Engineering Part A, 19(3), (doi:10.1089/ten.TEA.2012.0382)

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Abstract

Potential treatment strategies for the repair of spinal cord injury (SCI) currently favour a combinatorial approach incorporating several factors, including exogenous cell transplantation and biocompatible scaffolds. The use of scaffolds for bridging the gap at the injury site is very appealing although there has been little investigation into CNS neural cell interaction and survival on such scaffolds before implantation. Previously we demonstrated that aligned micro-grooves 12.5-25 µm wide on ε-polycaprolactone (PCL) promoted aligned neurite orientation and supported myelination. In this study we identify the appropriate substrate and its topographical features required for the design of a 3D scaffold intended for transplantation in SCI. Using an established myelinating culture system of dissociated spinal cord cells, recapitulating many of the features of the intact spinal cord, we demonstrate that astrocytes plated on the topography secrete soluble factors(s) that delay oligodendrocyte differentiation but do not prevent myelination. However, as myelination does occur after a further 10-12 days in culture this does not prevent the use of PCL as a scaffold material as part of a combined strategy for the repair of SCI.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Barnett, Professor Susan and Gadegaard, Professor Nikolaj and Donoghue, Dr Peter and Riehle, Dr Mathis and Boomkamp, Dr Stephanie
Authors: Donoghue, P.S., Lamond, R., Boomkamp, S.D., Tao, S., Gadegaard, N., Riehle, M.O., and Barnett, S.C.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Tissue Engineering Part A
Publisher:Mary Ann Liebert
ISSN:1937-3341
Published Online:06 December 2012
Copyright Holders:Copyright © 2012 Mary Ann Liebert Inc
First Published:First published in Tissue Engineering Part A 19(3):1-97
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
481921Micro Engineered Constructs for CNS repairSusan BarnettBiotechnology and Biological Sciences Research Council (BBSRC)BB/G004706/1III -IMMUNOLOGY