Smith, H., Doenhoff, M., Aitken, C., Bailey, W., Ji, M., Dawson, E., Gilis, H., Spence, G., Alexander, C. and van Gool, T. (2012) Comparison of schistosoma mansoni soluble cercarial antigens and soluble egg antigens for serodiagnosing schistosome infections. PLoS Neglected Tropical Diseases, 6(9), e1815. (doi: 10.1371/journal.pntd.0001815)
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Abstract
A Schistosoma mansoni cercarial antigen preparation (cercarial transformation fluid – SmCTF) was evaluated for detection of anti-schistosome antibodies in human sera in 4 collaborating laboratories. The performance of SmCTF was compared with that of S. mansoni egg antigens (SmSEA) in an indirect enzyme-immunoassay (ELISA) antigen assay, the latter being used routinely in 3 of the 4 participating laboratories to diagnose S. mansoni and S. haematobium infections. In the fourth laboratory the performance of SmCTF was compared with that of S. japonicum egg antigens (SjSEA) in ELISA for detection of anti-S. japonicum antibodies. In all 4 laboratories the results given by SmCTF in ELISA were very similar to those given by the antigen preparation routinely used in the respective laboratory to detect anti-schistosome antibodies in human infection sera. In so far as the ELISA results from SmCTF are thus so little different from those given by schistosome egg antigens and also cheaper to produce, the former is a potentially useful new diagnostic aid for schistosomiasis.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Smith, Prof Huw and Alexander, Miss Claire |
| Authors: | Smith, H., Doenhoff, M., Aitken, C., Bailey, W., Ji, M., Dawson, E., Gilis, H., Spence, G., Alexander, C., and van Gool, T. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Life Sciences College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing |
| Journal Name: | PLoS Neglected Tropical Diseases |
| Publisher: | Public Library of Science |
| ISSN: | 1935-2727 |
| ISSN (Online): | 1935-2735 |
| Published Online: | 13 September 2012 |
| Copyright Holders: | Copyright © 2012 The Authors |
| First Published: | First published in PLoS Neglected Tropical Diseases 6(9):e1815 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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