Inducible progenitor-derived wingless regulates adult midgut regeneration in drosophila

Cordero, J. B., Stefanatos, R. K., Scopelliti, A., Vidal, M. and Sansom, O. J. (2012) Inducible progenitor-derived wingless regulates adult midgut regeneration in drosophila. EMBO Journal, 31(19), pp. 3901-3917. (doi:10.1038/emboj.2012.248)

Cordero, J. B., Stefanatos, R. K., Scopelliti, A., Vidal, M. and Sansom, O. J. (2012) Inducible progenitor-derived wingless regulates adult midgut regeneration in drosophila. EMBO Journal, 31(19), pp. 3901-3917. (doi:10.1038/emboj.2012.248)

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Publisher's URL: http://dx.doi.org/10.1038/emboj.2012.248

Abstract

The ability to regenerate following stress is a hallmark of self-renewing tissues. However, little is known about how regeneration differs from homeostatic tissue maintenance. Here, we study the role and regulation of Wingless (Wg)/Wnt signalling during intestinal regeneration using the Drosophila adult midgut. We show that Wg is produced by the intestinal epithelial compartment upon damage or stress and it is exclusively required for intestinal stem cell (ISC) proliferation during tissue regeneration. Reducing Wg or downstream signalling components from the intestinal epithelium blocked tissue regeneration. Importantly, we demonstrate that Wg from the undifferentiated progenitor cell, the enteroblast, is required for Myc-dependent ISC proliferation during regeneration. Similar to young regenerating tissues, ageing intestines required Wg and Myc for ISC hyperproliferation. Unexpectedly, our results demonstrate that epithelial but not mesenchymal Wg is essential for ISC proliferation in response to damage, while neither source of the ligand is solely responsible for ISC maintenance and tissue self-renewal in unchallenged tissues. Therefore, fine-tuning Wnt results in optimal balance between the ability to respond to stress without negatively affecting organismal viability.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cordero, Dr Julia and Vidal, Dr Marcos and Stefanatos, Miss Rhoda and Sansom, Professor Owen and Scopelliti, Mr Alessandro
Authors: Cordero, J. B., Stefanatos, R. K., Scopelliti, A., Vidal, M., and Sansom, O. J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:EMBO Journal
Publisher:European Molecular Biology Organization
ISSN:0261-4189
ISSN (Online):1460-2075
Published Online:04 September 2012

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