Selective regulation of maize plasma membrane aquaporin trafficking and activity by the SNARE SYP121

Besserer, A., Burnotte, E., Bienert, G.P., Chevalier, A.S., Errachid, A., Grefen, C., Blatt, M.R. and Chaumont, F. (2012) Selective regulation of maize plasma membrane aquaporin trafficking and activity by the SNARE SYP121. Plant Cell, 24(8), pp. 3463-3481. (doi:10.1105/tpc.112.101758)

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Abstract

Plasma membrane intrinsic proteins (PIPs) are aquaporins facilitating the diffusion of water through the cell membrane. We previously showed that the traffic of the maize (Zea mays) PIP2;5 to the plasma membrane is dependent on the endoplasmic reticulum diacidic export motif. Here, we report that the post-Golgi traffic and water channel activity of PIP2;5 are regulated by the SNARE (for soluble N-ethylmaleimide-sensitive factor protein attachment protein receptor) SYP121, a plasma membrane resident syntaxin involved in vesicle traffic, signaling, and regulation of K+ channels. We demonstrate that the expression of the dominant-negative SYP121-Sp2 fragment in maize mesophyll protoplasts or epidermal cells leads to a decrease in the delivery of PIP2;5 to the plasma membrane. Protoplast and oocyte swelling assays showed that PIP2;5 water channel activity is negatively affected by SYP121-Sp2. A combination of in vitro (copurification assays) and in vivo (bimolecular fluorescence complementation, Förster resonance energy transfer, and yeast split-ubiquitin) approaches allowed us to demonstrate that SYP121 and PIP2;5 physically interact. Together with previous data demonstrating the role of SYP121 in regulating K+ channel trafficking and activity, these results suggest that SYP121 SNARE contributes to the regulation of the cell osmotic homeostasis.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Blatt, Professor Michael and Grefen, Dr Christopher
Authors: Besserer, A., Burnotte, E., Bienert, G.P., Chevalier, A.S., Errachid, A., Grefen, C., Blatt, M.R., and Chaumont, F.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Plant Cell
ISSN:1040-4651
ISSN (Online):1532-298X
Published Online:01 August 2012

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