Yin, Y., Borges, G., Sakuta, M., Crozier, A. and Ashihara, H. (2012) Effect of phosphate deficiency on the content and biosynthesis of anthocyanins and the expression of related genes in suspension-cultured grape (Vitis sp.) cells. Plant Physiology and Biochemistry, 55, pp. 77-84. (doi: 10.1016/j.plaphy.2012.03.009)
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Abstract
The effect of phosphate (Pi) starvation on anthocyanin biosynthesis was examined in suspension-cultured grape cells derived from Bailey Alicante A grapes. Cyanindin-3-O-glucoside, peonidin-3-O-glucoside, cyanidin-3-O-(6-O״-p-coumaroyl)-glucoside and peonidin-3-O-(6-O״-p-coumaroyl)-glucoside were detected in cells in both the complete (+Pi)- and Pi-deficient Murashige-Skoog (-Pi) medium but accumulation was much more substantial cells in -Pi cultures. Highest incorporation of [ring-(14)C]phenylalanine into anthocyanins was found at days 3-14 in the cells in -Pi medium. Time-course expression profiles of anthocyanin biosynthetic enzyme genes and a regulatory gene were examined after cells were transferred to the +Pi and -Pi medium. The transcript levels of UFGT and VvmybA1 in -Pi cells were higher than in +Pi cells 24 h after transfer coinciding with the start of anthocyanin accumulation in -Pi cells.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Borges, Dr Gina and Crozier, Professor Alan |
Authors: | Yin, Y., Borges, G., Sakuta, M., Crozier, A., and Ashihara, H. |
College/School: | College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing |
Journal Name: | Plant Physiology and Biochemistry |
Journal Abbr.: | Plant Physiol. Biochem. |
ISSN: | 0981-9428 |
ISSN (Online): | 1873-2690 |
Published Online: | 07 April 2012 |
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