Using nanotopography and metabolomics to identify biochemical effectors of multipotency

Tsimbouri, P.M. , McMurray, R.J., Burgess, K.V., Alakpa, E.V., Reynolds, P., Murawski, K., Kingham, E., Oreffo, R.O.C., Gadegaard, N. and Dalby, M.J. (2012) Using nanotopography and metabolomics to identify biochemical effectors of multipotency. ACS Nano, 6(11), pp. 10239-10249. (doi:10.1021/nn304046m)

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Abstract

It is emerging that mesenchymal stem cell (MSC) metabolic activity may be a key regulator of multipotency. The metabolome represents a “snapshot” of the stem cell phenotype, and therefore metabolic profiling could, through a systems biology approach, offer and highlight critical biochemical pathways for investigation. To date, however, it has remained difficult to undertake unbiased experiments to study MSC multipotency in the absence of strategies to retain multipotency without recourse to soluble factors that can add artifact to experiments. Here we apply a nanotopographical systems approach linked to metabolomics to regulate plasticity and demonstrate rapid metabolite reorganization, allowing rational selection of key biochemical targets of self-renewal (ERK1/2, LDL, and Jnk). We then show that these signaling effectors regulate functional multipotency.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Tsimbouri, Dr Penelope and Dalby, Professor Matthew and Alakpa, Miss Enateri and Reynolds, Mr Paul and Burgess, Dr Karl and Gadegaard, Professor Nikolaj
Authors: Tsimbouri, P.M., McMurray, R.J., Burgess, K.V., Alakpa, E.V., Reynolds, P., Murawski, K., Kingham, E., Oreffo, R.O.C., Gadegaard, N., and Dalby, M.J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering > School of Engineering > Biomedical Engineering
College of Science and Engineering > School of Geographical and Earth Sciences
Journal Name:ACS Nano
Publisher:American Chemical Society
ISSN:1936-0851
ISSN (Online):1936-086X
Published Online:16 October 2012

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