Christie, J.M. , Hitomi, K., Arvai, A.S., Hartfield, K.A., Mettlen, M., Pratt, A.J., Tainer, J.A. and Getzoff, E.D. (2012) Structural tuning of the fluorescent protein iLOV for improved photostability. Journal of Biological Chemistry, 287(26), pp. 22295-22304. (doi: 10.1074/jbc.M111.318881)
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Abstract
Background: iLOV is a fluorescent flavoprotein engineered from the plant blue light receptor phototropin. <p/>Results: Structures reveal altered protein-chromophore interactions within the flavin-binding cavity of iLOV when compared with its progenitors. Directed evolution further anchored the chromophore to increase iLOV photostability by an order of magnitude. <p/>Conclusion: Improving iLOV photostability by constraining its fluorophore establishes a framework for fine-tuning fluorescence. <p/>Significance: Enhanced photostability increases iLOV utility as an oxygen-independent fluorescent reporter.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Christie, Professor John |
Authors: | Christie, J.M., Hitomi, K., Arvai, A.S., Hartfield, K.A., Mettlen, M., Pratt, A.J., Tainer, J.A., and Getzoff, E.D. |
College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
Journal Name: | Journal of Biological Chemistry |
Publisher: | American Society for Biochemistry and Molecular Biology, Inc. |
ISSN: | 0021-9258 |
Published Online: | 09 May 2012 |
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