Lentiviral manipulation of gene expression in human adult and embryonic stem cells

Clements, M.O., Godfrey, A., Crossley, J., Wilson, S.J. , Takeuchi, Y. and Boshoff, C. (2006) Lentiviral manipulation of gene expression in human adult and embryonic stem cells. Tissue Engineering, 12(7), pp. 1741-1751. (doi:10.1089/ten.2006.12.1741)

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Abstract

Human stem cells could revolutionize the field of medicine by providing a diverse range of cell types for tissue replacement therapies and drug discovery. To achieve this goal, genetic tools need to be optimized and developed for controlling and manipulating stem cells ex vivo. Here we describe a lentiviral delivery system capable of high infection rates in human mesenchymal and embryonic stem cells. The lentiviral backbone was modified to express mono- and bi-cistronic transgenes and was also used to deliver short hairpin ribonucleic acid for specific silencing of gene expression in human stem cells. We show that lentiviral transduction can be used to alter gene expression without altering the genes' ability to differentiate in vitro. These vectors will enable rapid analysis of gene function in stem cells and permit the generation of knock-in / knock-out models of human disease in the rapidly developing field of gene therapy.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Wilson, Dr Sam
Authors: Clements, M.O., Godfrey, A., Crossley, J., Wilson, S.J., Takeuchi, Y., and Boshoff, C.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Tissue Engineering
ISSN:1076-3279
ISSN (Online):1557-8690
Copyright Holders:Copyright © 2006 Mary Ann Liebert Inc.
First Published:First published in Tissue Engineering 12(7):1741-1751
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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