Clements, M.O., Godfrey, A., Crossley, J., Wilson, S.J. , Takeuchi, Y. and Boshoff, C. (2006) Lentiviral manipulation of gene expression in human adult and embryonic stem cells. Tissue Engineering, 12(7), pp. 1741-1751. (doi: 10.1089/ten.2006.12.1741)
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Abstract
Human stem cells could revolutionize the field of medicine by providing a diverse range of cell types for tissue replacement therapies and drug discovery. To achieve this goal, genetic tools need to be optimized and developed for controlling and manipulating stem cells ex vivo. Here we describe a lentiviral delivery system capable of high infection rates in human mesenchymal and embryonic stem cells. The lentiviral backbone was modified to express mono- and bi-cistronic transgenes and was also used to deliver short hairpin ribonucleic acid for specific silencing of gene expression in human stem cells. We show that lentiviral transduction can be used to alter gene expression without altering the genes' ability to differentiate in vitro. These vectors will enable rapid analysis of gene function in stem cells and permit the generation of knock-in / knock-out models of human disease in the rapidly developing field of gene therapy.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Wilson, Professor Sam |
Authors: | Clements, M.O., Godfrey, A., Crossley, J., Wilson, S.J., Takeuchi, Y., and Boshoff, C. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
Journal Name: | Tissue Engineering |
ISSN: | 1076-3279 |
ISSN (Online): | 1557-8690 |
Copyright Holders: | Copyright © 2006 Mary Ann Liebert Inc. |
First Published: | First published in Tissue Engineering 12(7):1741-1751 |
Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher |
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