Expression of membrane-associated proteins within single emulsion cell facsimiles

Chanasakulniyom, M., Martino, C., Paterson, D., Horsfall, L., Rosser, S. and Cooper, J. (2012) Expression of membrane-associated proteins within single emulsion cell facsimiles. Analyst, 137(13), pp. 2939-2943. (doi:10.1039/C2AN35047E)

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Abstract

MreB is a structural membrane-associated protein which is one of the key components of the bacterial cytoskeleton. Although it plays an important role in shape maintenance of rod-like bacteria, the understanding of its mechanism of action is still not fully understood. This study shows how segmented flow and microdroplet technology can be used as a new tool for biological in vitro investigation of this protein. In this paper, we demonstrate cell-free expression in a single emulsion system to express red fluorescence protein (RFP) and MreB linked RFP (MreB–RFP). We follow the aggregation and localisation of the fusion protein MreB–RFP in this artificial cell-like environment. The expression of MreB–RFP in single emulsion droplets leads to the formation of micrometer-scale protein patches distributed at the water/oil interface.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Rosser, Professor Susan and Horsfall, Dr Louise and Cooper, Professor Jonathan
Authors: Chanasakulniyom, M., Martino, C., Paterson, D., Horsfall, L., Rosser, S., and Cooper, J.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Analyst
ISSN:0003-2654
Published Online:05 March 2012

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