Intrahost evolutionary dynamics of canine influenza virus in naive and partially immune dogs

Hoelzer, K., Murcia, P.R. , Baillie, G.J., Wood, J.L.N., Metzger, S.M., Osterrieder, N., Dubovi, E.J., Holmes, E.C. and Parrish, C.R. (2010) Intrahost evolutionary dynamics of canine influenza virus in naive and partially immune dogs. Journal of Virology, 84(10), pp. 5329-5335. (doi: 10.1128/​JVI.02469-09)

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Abstract

The patterns and dynamics of evolution in acutely infecting viruses within individual hosts are largely unknown. To this end, we investigated the intrahost variation of canine influenza virus (CIV) during the course of experimental infections in naïve and partially immune dogs and in naturally infected dogs. Tracing sequence diversity in the gene encoding domain 1 of the hemagglutinin (HA1) protein over the time course of infection provided information on the patterns and processes of intrahost viral evolution and revealed some of the effects of partial host immunity. Viral populations sampled on any given day were generally characterized by mean pairwise genetic diversities between 0.1 and 0.2% and by mutational spectra that changed considerably on different days. Some observed mutations may have affected antigenicity or host range, including reversions of CIV host-associated mutations. Patterns of sequence diversity differed between naïve and vaccinated dogs, with some presumably antigenic mutations transiently reaching high frequency in the latter. CIV populations are therefore characterized by the rapid generation and clearance of genetic diversity. Potentially advantageous mutations arise readily during the course of single infections and may give rise to antigenic escape or host range variants.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Murcia, Professor Pablo
Authors: Hoelzer, K., Murcia, P.R., Baillie, G.J., Wood, J.L.N., Metzger, S.M., Osterrieder, N., Dubovi, E.J., Holmes, E.C., and Parrish, C.R.
College/School:College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:Journal of Virology
Journal Abbr.:J. Virol.
ISSN:0022-538X
ISSN (Online):1098-5514
Published Online:10 March 2010

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
579921Intra and inter-host evolution of equine and swine influenza virusesPablo MurciaWellcome Trust (WELLCOME)088494/Z/09/ZIII - CENTRE FOR VIRUS RESEARCH