Brewer, L.R., Davidson, J.C., Balch, J.W., and Carrano, A.V. (1995) Three-dimensional imaging of DNA fragments during electrophoresis using a confocal detector. Electrophoresis, 16 (1). pp. 1846-1850. ISSN 0173-0835 (doi:10.1002/elps.11501601303)
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Publisher's URL: http://dx.doi.org/10.1002/elps.11501601303
We have measured the three-dimensional distribution of DNA fragments within an electrophoretic band confined by a glass microchannel. The measurements were made using a confocal microscope and a photon-counting photo-multiplier detector. A DNA sequencing standard was loaded into glass micro-channel plates containing polyacrylamide gel. The measurements were made by scanning the plates in three dimensions using a mechanical stage under computer control, while electrophoresis was taking place. We found that the distribution of DNA was the same for all the bands measured in the sequencing ladder with an approximate Gaussian distribution along all three axes. These measurements are important to understand what physical forces shape electrophoretic bands confined by a channel and they also aid in the design of high throughput DNA sequencers.
|Glasgow Author(s):||Brewer, Dr Laurence|
|Authors:||Brewer, L.R., Davidson, J.C., Balch, J.W., and Carrano, A.V.|
|College/School:||College of Science and Engineering > School of Physics and Astronomy|
|Published Online:||14 April 2005|