Phenotypic suppression of the Drosophila mitochondrial disease-like mutant tko25t by duplication of the mutant gene in its natural chromosomal context

Kemppainen, E., Fernández-Ayala, D. J. M., Galbraith, L. C.A., O'Dell, K. M.C. and Jacobs, H. T. (2009) Phenotypic suppression of the Drosophila mitochondrial disease-like mutant tko25t by duplication of the mutant gene in its natural chromosomal context. Mitochondrion, 9(5), pp. 353-363. (doi: 10.1016/j.mito.2009.07.002)

Abstract

A mutation in the Drosophilagenetechnical knockout (tko25t), encoding mitoribosomal protein S12, phenocopies human mitochondrialdisease. We isolated three spontaneous X-dominant suppressors of tko25t (designated Weeble), exhibiting almost wild-type phenotype and containing overlapping segmental duplications including the mutant allele, plus a second mitoribosomal protein gene, mRpL14. Ectopic, expressed copies of tko25t and mRpL14 conferred no phenotypicsuppression. When placed over a null allele of tko, Weeble retained the mutant phenotype, even in the presence of additional transgenic copies of tko25t. Increased mutantgene dosage can thus compensate the mutant phenotype, but only when located in its normal chromosomalcontext.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: O'Dell, Professor Kevin and Galbraith, Dr Laura
• Authors: Kemppainen, E., Fernández-Ayala, D. J. M., Galbraith, L. C.A., O'Dell, K. M.C., and Jacobs, H. T.
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences; College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
• Journal Name: Mitochondrion
• Publisher: Elsevier
• ISSN: 1567-7249
• ISSN (Online): 1872-8278
• Published Online: 17 July 2009