A miniaturized bioreactor system for the evaluation of cell interaction with designed substrates in perfusion culture

Sun, T., Donoghue, P.S., Higginson, J.R., Gadegaard, N. , Barnett, S.C. and Riehle, M.O. (2013) A miniaturized bioreactor system for the evaluation of cell interaction with designed substrates in perfusion culture. Journal of Tissue Engineering and Regenerative Medicine, 6(S3), S4-S14. (doi:10.1002/term.510)

Sun, T., Donoghue, P.S., Higginson, J.R., Gadegaard, N. , Barnett, S.C. and Riehle, M.O. (2013) A miniaturized bioreactor system for the evaluation of cell interaction with designed substrates in perfusion culture. Journal of Tissue Engineering and Regenerative Medicine, 6(S3), S4-S14. (doi:10.1002/term.510)

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Abstract

In tissue engineering, the chemical and topographical cues within three-dimensional (3D) scaffolds are normally tested using static cell cultures but applied directly to tissue cultures in perfusion bioreactors. As human cells are very sensitive to the changes of culture environment, it is essential to evaluate the performance of any chemical, and topographical cues in a perfused environment before they are applied to tissue engineering. Thus the aim of this research was to bridge the gap between static and perfusion cultures by addressing the effect of perfusion on cell cultures within 3D scaffolds. For this we developed a scale down bioreactor system, which allows to evaluate the effectiveness of various chemical and topographical cues incorporated into our previously developed tubular ε-polycaprolactone scaffold under perfused conditions. Investigation of two exemplary cell types (fibroblasts and cortical astrocytes) using the miniaturized bioreactor indicated that: (1) quick and firm cell adhesion in 3D scaffold was critical for cell survival in perfusion culture compared with static culture, thus cell seeding procedures for static cultures might not be applicable. Therefore it was necessary to re-evaluate cell attachment on different surfaces under perfused conditions before a 3D scaffold was applied for tissue cultures, (2) continuous medium perfusion adversely influenced cell spread and survival, which could be balanced by intermittent perfusion, (3) micro-grooves still maintained its influences on cell alignment under perfused conditions, while medium perfusion demonstrated additional influence on fibroblast alignment but not on astrocyte alignment on grooved substrates. This research demonstrated that the mini-bioreactor system is crucial for the development of functional scaffolds with suitable chemical and topographical cues by bridging the gap between static culture and perfusion culture.

Item Type:Articles
Keywords:Miniaturized bioreactor, perfusion culture, astrocytes, fibroblasts, tissue engineering
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Barnett, Professor Susan and Sun, Dr Tao and Higginson, Dr Jennifer and Gadegaard, Professor Nikolaj and Donoghue, Dr Peter and Riehle, Dr Mathis
Authors: Sun, T., Donoghue, P.S., Higginson, J.R., Gadegaard, N., Barnett, S.C., and Riehle, M.O.
Subjects:Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
T Technology > TK Electrical engineering. Electronics Nuclear engineering
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Journal of Tissue Engineering and Regenerative Medicine
Publisher:John Wiley & Sons Ltd
ISSN:1932-6254
ISSN (Online):1932-7005
Published Online:13 December 2011
Copyright Holders:Copyright © 2011 John Wiley and Sons Ltd
First Published:First published in Journal of Tissue Engineering and Regenerative Medicine
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
481921Micro Engineered Constructs for CNS repairSusan BarnettBiotechnology and Biological Sciences Research Council (BBSRC)BB/G004706/1III -IMMUNOLOGY