Lowndes, N.F., McInerny, C.J., Johnson, A.L., Fantes, P.A, and Johnston, L.H. (1992) Control of DNA synthesis genes in fission yeast by the cell-cycle gene cdc10+. Nature, 355 (6359). pp. 449-453. ISSN 0028-0836 (doi:10.1038/355449a0)
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In the budding yeast Saccharomyces cerevisiae, cell-cycle control over DNA synthesis occurs partly through the coordinate expression in late G1 phase of many, if not all, of the genes required for DNA synthesis. A cis-acting hexamer element ACGCGT (an MluI restriction site) is responsible for coordinating transcriptional regulation of these genes at the G1/S phase boundary and we have identified a binding activity, DSC1, that recognizes these sequences in a cell-cycle-dependent manner. In the distantly related fission yeast Schizosaccharomyces pombe, only one of the known DNA synthesis genes, cdc22+, which encodes a subunit of ribonucleotide reductase, is periodically expressed in late G1 (ref. 6). The promoter region of cdc22+ has two MluI sites and five related sequences, suggesting that similar controls over DNA synthesis genes could occur in fission yeast. We report here a binding activity in fission yeast that is very similar to DSC1 in budding yeast. We also show that the fission yeast cdc10+ gene product, which is required for Start and entry into S phase, is a component of this binding activity.
|Item Type:||Article (Letter)|
|Glasgow Author(s):||McInerny, Dr Christopher|
|Authors:||Lowndes, N.F., McInerny, C.J., Johnson, A.L., Fantes, P.A, and Johnston, L.H.|
|College/School:||College of Medical Veterinary and Life Sciences > School of Life Sciences > Life Sciences Biomolecular Science|