Positive and negative roles for cdc10 in cell cycle gene expression

McInerny, C.J., Kersey, P.J., Creanor, J. and Fantes, P.A. (1995) Positive and negative roles for cdc10 in cell cycle gene expression. Nucleic Acids Research, 23(23), pp. 4761-4768. (doi:10.1093/nar/23.23.4761)

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In this paper we describe properties of the cdc10-C4 mutant of the fission yeast Schizosaccharomyces pombe. The cdc10+ gene encodes a component of the DSC1Sp/MBF transcription complex, which is required for cell-cycle regulated expression at G1-S of several genes via cis-acting MCB (MIuI cell cycle box) elements. At permissive temperatures cdc10-C4 causes expression of MCB-regulated genes through the whole cell cycle, which in asynchronously dividing cells is manifested in overall higher expression levels. This overexpression phenotype is cold sensitive: in cdc10-C4 cells, MCB genes are expressed offprogressively higher levels at lower temperatures. In heterozygous cdc10-C4/cdc10+ diploid strains, MCB-regulated genes are not overexpressed, suggesting that loss, rather than alteration, of function of the cdc10-C4 gene product is the reason for unregulated target gene expression. Consistent with this, the cdc10-C4 mutant allele is known to encode a truncated protein. We have also overexpressed the region of the cdc10 protein absent in cdc10-C4 under the control of an inducible promoter. This induces a G1 delay, and additionally causes a reduction of the overexpression of MCB genes in cdc10-C4 strains. These results suggest that DSC1Sp/MBF represses, as well as activates, MCB gene expression during the cell cycle.

Item Type:Articles
Glasgow Author(s) Enlighten ID:McInerny, Dr Christopher
Authors: McInerny, C.J., Kersey, P.J., Creanor, J., and Fantes, P.A.
College/School:College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:Nucleic Acids Research
Journal Abbr.:Nucl. Acids Res.
ISSN (Online):1362-4954

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