McInerny, C.J., Kersey, P.J., Creanor, J., and Fantes, P.A. (1995) Positive and negative roles for cdc10 in cell cycle gene expression. Nucleic Acids Research, 23 (23). pp. 4761-4768. ISSN 0305-1048 (doi:10.1093/nar/23.23.4761)
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In this paper we describe properties of the cdc10-C4 mutant of the fission yeast Schizosaccharomyces pombe. The cdc10+ gene encodes a component of the DSC1Sp/MBF transcription complex, which is required for cell-cycle regulated expression at G1-S of several genes via cis-acting MCB (MIuI cell cycle box) elements. At permissive temperatures cdc10-C4 causes expression of MCB-regulated genes through the whole cell cycle, which in asynchronously dividing cells is manifested in overall higher expression levels. This overexpression phenotype is cold sensitive: in cdc10-C4 cells, MCB genes are expressed offprogressively higher levels at lower temperatures. In heterozygous cdc10-C4/cdc10+ diploid strains, MCB-regulated genes are not overexpressed, suggesting that loss, rather than alteration, of function of the cdc10-C4 gene product is the reason for unregulated target gene expression. Consistent with this, the cdc10-C4 mutant allele is known to encode a truncated protein. We have also overexpressed the region of the cdc10 protein absent in cdc10-C4 under the control of an inducible promoter. This induces a G1 delay, and additionally causes a reduction of the overexpression of MCB genes in cdc10-C4 strains. These results suggest that DSC1Sp/MBF represses, as well as activates, MCB gene expression during the cell cycle.
|Glasgow Author(s):||McInerny, Dr Christopher|
|Authors:||McInerny, C.J., Kersey, P.J., Creanor, J., and Fantes, P.A.|
|College/School:||College of Medical Veterinary and Life Sciences > School of Life Sciences > Life Sciences Biomolecular Science|
|Journal Name:||Nucleic Acids Research|
|Journal Abbr.:||Nucl. Acids Res.|