Positive and negative roles for cdc10 in cell cycle gene expression

McInerny, C.J. , Kersey, P.J., Creanor, J. and Fantes, P.A. (1995) Positive and negative roles for cdc10 in cell cycle gene expression. Nucleic Acids Research, 23(23), pp. 4761-4768. (doi: 10.1093/nar/23.23.4761)

Abstract

In this paper we describe properties of the cdc10-C4 mutant of the fission yeast Schizosaccharomyces pombe. The cdc10⁺ gene encodes a component of the DSC1^Sp/MBF transcription complex, which is required for cell-cycle regulated expression at G1-S of several genes via cis-acting MCB (MIuI cell cycle box) elements. At permissive temperatures cdc10-C4 causes expression of MCB-regulated genes through the whole cell cycle, which in asynchronously dividing cells is manifested in overall higher expression levels. This overexpression phenotype is cold sensitive: in cdc10-C4 cells, MCB genes are expressed offprogressively higher levels at lower temperatures. In heterozygous cdc10-C4/cdc10⁺ diploid strains, MCB-regulated genes are not overexpressed, suggesting that loss, rather than alteration, of function of the cdc10-C4 gene product is the reason for unregulated target gene expression. Consistent with this, the cdc10-C4 mutant allele is known to encode a truncated protein. We have also overexpressed the region of the cdc10 protein absent in cdc10-C4 under the control of an inducible promoter. This induces a G1 delay, and additionally causes a reduction of the overexpression of MCB genes in cdc10-C4 strains. These results suggest that DSC1^Sp/MBF represses, as well as activates, MCB gene expression during the cell cycle.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: McInerny, Dr Chris
• Authors: McInerny, C.J., Kersey, P.J., Creanor, J., and Fantes, P.A.
• College/School: College of Medical Veterinary and Life Sciences > School of Life Sciences; College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
• Journal Name: Nucleic Acids Research
• Journal Abbr.: Nucl. Acids Res.
• ISSN: 0305-1048
• ISSN (Online): 1362-4954