Integrated microspectrometer for fluorescence based analysis in a microfluidic format

Hu, Z., Glidle, A., Ironside, C. N., Sorel, M., Strain, M. J., Cooper, J. and Yin, H. (2012) Integrated microspectrometer for fluorescence based analysis in a microfluidic format. Lab on a Chip, 12(16), pp. 2850-2857. (doi:10.1039/C2LC40169J)

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Publisher's URL: http://dx.doi.org/10.1039/C2LC40169J

Abstract

We have demonstrated a monolithic integrated arrayed waveguide grating (AWG) microspectrometer microfluidic platform capable of fluorescence spectroscopic analysis. The microspectrometer in this proof of concept study has a small (1 cm × 1 cm) footprint and 8 output channels centred on different wavelengths. We show that the signals from the output channels detected on a camera chip can be used to recreate the complete fluorescence spectrum of an analyte. By making fluorescence measurements of (i) mixed quantum dot solutions, (ii) an organic fluorophore (Cy5) and (iii) the propidium iodide (PI)-DNA assay, we illustrate the unique advantages of the AWG platform for simultaneous, quantitative multiplex detection and its capability to detect small spectroscopic shifts. Although the current system is designed for fluorescence spectroscopic analysis, in principle, it can be implemented for other types of analysis, such as Raman spectroscopy. Fabricated using established semiconductor industry methods, this miniaturised platform holds great potential to create a handheld, low cost biosensor with versatile detection capability.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Yin, Professor Huabing and Sorel, Professor Marc and Ironside, Professor Charles and Glidle, Dr Andrew and Cooper, Professor Jonathan and Strain, Dr Michael
Authors: Hu, Z., Glidle, A., Ironside, C. N., Sorel, M., Strain, M. J., Cooper, J., and Yin, H.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering
Journal Name:Lab on a Chip
Publisher:Royal Society of Chemistry
ISSN:1473-0197
ISSN (Online):1473-0189
Published Online:03 April 2012

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