Characterisation and differentiation potential of bone marrow derived canine mesenchymal stem cells

Hodgkiss-Geere, H.M., Argyle, D.J., Corcoran, B.M., Whitelaw, B., Milne, E., Bennett, D.B. and Argyle, S.A. (2013) Characterisation and differentiation potential of bone marrow derived canine mesenchymal stem cells. Veterinary Journal, 194(3), pp. 361-368. (doi: 10.1016/j.tvjl.2012.05.011)

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Abstract

Mesenchymal stem cells (MSCs) have potential for use in regenerative therapeutics, since they are capable of multi-lineage differentiation. In this study, primary canine MSCs (cMSCs) were isolated from bone marrow aspirates and characterised using marker expression and morphology. cMSCs expressed CD44 and STRO-1, but not CD34 or CD45. Morphologically, cMSCs were similar to previously described MSCs and were capable of chondrocyte differentiation towards articular type cartilage, characterised by increased collagen type II vs. collagen type I expression and expression of Sox-9. cMSCs demonstrated no significant alterations in marker profiles and failed to differentiate into cardiomyocytes in response to a cardiac differentiation protocol or when co-cultured with canine cardiac stem cells. The study indicated that cMSCs can be derived readily from bone marrow and are capable of differentiation into articular cartilage, but appear to have limited ability to differentiate into cardiomyocytes using current protocols.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Bennett, Professor David
Authors: Hodgkiss-Geere, H.M., Argyle, D.J., Corcoran, B.M., Whitelaw, B., Milne, E., Bennett, D.B., and Argyle, S.A.
College/School:College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:Veterinary Journal
Publisher:Elsevier
ISSN:1090-0233
Published Online:01 December 2012

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