Autotransporter passenger domain secretion requires a hydrophobic cavity at the extracellular entrance of the β-domain pore

Zhai, Y. et al. (2011) Autotransporter passenger domain secretion requires a hydrophobic cavity at the extracellular entrance of the β-domain pore. Biochemical Journal, 435(3), pp. 577-587. (doi: 10.1042/BJ20101548)

Abstract

Whooping cough (pertussis) is a highly contagious acute respiratory illness of humans caused by the Gram-negative bacterial pathogen Bordetella pertussis. The AT (autotransporter) BrkA (Bordetella serum-resistance killing protein A) is an important B. pertussis virulence factor that confers serum resistance and mediates adherence. In the present study, we have solved the crystal structure of the BrkA β-domain at 3 Å (1 Å=0.1 nm) resolution. Special features are a hairpin-like structure formed by the external loop L4, which is observed fortuitously sitting inside the pore of the crystallographic adjacent β-domain, and a previously undiscovered hydrophobic cavity formed by patches on loop L4 and β-strands S5 and S6. This adopts a ubiquitous structure characteristic of all AT β-domains. Mutagenesis studies have demonstrated that the hairpin-like structure and hydrophobic cavity are crucial for BrkA passenger domain (virulence effector) translocation. This structure helps in understanding the molecular mechanism of AT assembly and secretion and provides a potential target for anti-pertussis drug design.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Roszak, Dr Aleksander and Isaacs, Professor Neil
• Authors: Zhai, Y., Zhang, K., Huo, Y., Zhu, Y., Zhou, Q., Lu, J., Black, I., Pang, X., Roszak, A.W., Zhang, X., Isaacs, N.W., and Sun, F.
• College/School: College of Science and Engineering > School of Chemistry
• Journal Name: Biochemical Journal
• ISSN: 0264-6021
• ISSN (Online): 1470-8728
• Published Online: 10 February 2011