Trapping and imaging of micron-sized embryos using dielectrophoresis

Khoshmanesh, K., Kiss, N., Nahavandi, S., Evans, C.W., Cooper, J.M. , Williams, D.E. and Wlodkowic, D. (2011) Trapping and imaging of micron-sized embryos using dielectrophoresis. Electrophoresis, 32(22), pp. 3129-3132. (doi:10.1002/elps.201100160)

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Abstract

Development of dielectrophoretic (DEP) arrays for real-time imaging of embryonic organisms is described. Microelectrode arrays were used for trapping both embryonated eggs and larval stages of Antarctic nematode Panagrolaimus davidi. Ellipsoid single-shell model was also applied to study the interactions between DEP fields and developing multicellular organisms. This work provides proof-of-concept application of chip-based technologies for the analysis of individual embryos trapped under DEP force.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cooper, Professor Jonathan
Authors: Khoshmanesh, K., Kiss, N., Nahavandi, S., Evans, C.W., Cooper, J.M., Williams, D.E., and Wlodkowic, D.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Electrophoresis
ISSN:0173-0835
Published Online:27 July 2011

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