The structure of an orthorhombic crystal form of a 'forced reduced' thiol peroxidase reveals lattice formation aided by the presence of the affinity tag

Beckham, K. S.H., Byron, O. , Roe, A. J. and Gabrielsen, M. (2012) The structure of an orthorhombic crystal form of a 'forced reduced' thiol peroxidase reveals lattice formation aided by the presence of the affinity tag. Acta Crystallographica. Section F: Structural Biology and Crystallization Communications, 68(5), pp. 522-526. (doi:10.1107/S1744309112011487) (PMID:22691780) (PMCID:PMC3374505)

Beckham, K. S.H., Byron, O. , Roe, A. J. and Gabrielsen, M. (2012) The structure of an orthorhombic crystal form of a 'forced reduced' thiol peroxidase reveals lattice formation aided by the presence of the affinity tag. Acta Crystallographica. Section F: Structural Biology and Crystallization Communications, 68(5), pp. 522-526. (doi:10.1107/S1744309112011487) (PMID:22691780) (PMCID:PMC3374505)

[img] Text
66789.pdf

21MB

Abstract

Thiol peroxidase (Tpx) is an atypical 2-Cys peroxiredoxin, which has been suggested to be important for cell survival and virulence in Gram-negative pathogens. The structure of a catalytically inactive version of this protein in an orthorhombic crystal form has been determined by molecular replacement. Structural alignments revealed that Tpx is conserved. Analysis of the crystal packing shows that the linker region of the affinity tag is important for formation of the crystal lattice.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Gabrielsen, Dr Mads and Roe, Professor Andrew and Byron, Professor Olwyn
Authors: Beckham, K. S.H., Byron, O., Roe, A. J., and Gabrielsen, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Acta Crystallographica. Section F: Structural Biology and Crystallization Communications
Publisher:International Union of Crystallography
ISSN:1744-3091
Copyright Holders:Copyright © 2012 International Union of Crystallography
First Published:First published in Acta Crystallographica. Section F: Structural Biology and Crystallization Communications 68(5):522-526
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
491441A biochemical and molecular analysis of the YhaO membrane protein in Escherichia coli O157:H7Andrew RoeBiotechnology and Biological Sciences Research Council (BBSRC)BB/G011389/1III - BACTERIOLOGY