E2F-7: a distinctive E2F family member with an unusual organization of DNA-binding domains

Logan, N., Delavaine, L., Graham, A., Reilly, C., Wilson, J., Brummelkamp, T. R., Hijmans, E. M., Bernards, R. and La Thangue, N. B. (2004) E2F-7: a distinctive E2F family member with an unusual organization of DNA-binding domains. Oncogene, 23(30), pp. 5138-5150. (doi: 10.1038/sj.onc.1207649) (PMID:15133492)

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Abstract

The E2F family of transcription factors play an important role in regulating cell cycle progression. We report here the characterization and functional properties of a new member of the human E2F family, referred to as E2F-7. E2F-7 has two separate DNA-binding domains, a feature that distinguishes E2F-7 from other mammalian E2F proteins, but resembling the organization of recently isolated E2F-like proteins from Arabidopsis. E2F-7 binds to DNA independently of a DP partner and delays cell cycle progression. Interestingly, E2F-7 modulates the transcription properties of other E2F proteins. A mutational analysis indicates that the integrity of both DNA-binding domains is required for cell cycle delay and transcriptional modulation. Biochemical results and protein modelling studies suggest that in binding to DNA interactions occur between the two DNA-binding domains, most probably as a homodimer, thereby mimicking the organization of an E2F/DP heterodimer. These structural and functional properties of E2F-7 imply a unique role in regulating cellular proliferation.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:McKenna, Mrs Anne and Logan, Miss Nicola
Authors: Logan, N., Delavaine, L., Graham, A., Reilly, C., Wilson, J., Brummelkamp, T. R., Hijmans, E. M., Bernards, R., and La Thangue, N. B.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
Journal Name:Oncogene
Publisher:Nature Publishing Group
ISSN:0950-9232
ISSN (Online):1476-5594
Published Online:10 May 2004

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
277581Molecular and Functional characterisation of the E2F Pathway of Growth ControlNicholas La ThangueMedical Research Council (MRC)G9400953Biochemistry & Cell Biology