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Simultaneous measurement of albumin and fibrinogen synthetic rates in normal fasted subjects

McMillan, D.C., Slater, C., Preston, T., Falconer, J.S., and Fearon, K.C.H. (1996) Simultaneous measurement of albumin and fibrinogen synthetic rates in normal fasted subjects. Nutrition, 12 (9). pp. 602-607. ISSN 0899-9007 (doi:10.1016/S0899-9007(96)00172-4)

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Abstract

Albumin and fibrinogen synthesis appear to account for the majority of protein exported by the liver and therefore make a substantial contribution to that of whole-body protein synthesis. However, data on the protein syntheticrates of albumin and fibrinogen in normalsubjects are limited. Albumin and fibrinogensyntheticrates were measured simultaneously over a 120-min period in normalsubjects (n = 6) by using a flooding dose of 2H5-phenylalanine. Tracer incorporation into proteins was measured by gas chromatography/mass spectrometry. Body mass index, circulating concentrations of insulin, albumin, fibrinogen, C-reactive protein, and plasma volumes of the subjects were all within the normal reference range. There was a small and transient rise in circulating insulin concentrations following the flooding dose of phenylalanine. The median fractional syntheticrate and absolute syntheticrate for albumin was 10.3%/d and 208 mg · kg−1 · d−1, respectively. The median fractional syntheticrate and absolute syntheticrate for fibrinogen was 19.5%/d and 28 mg · kg−1 · d−1. In the context of the current interest in manipulating the inflammatory response of patients with various disease states, we introduce the concept of an acute phase protein quotient (APPQ). The APPQ is defined as the absolute rate of fibrinogen synthesis divided by that of albumin. In this group of normalsubjects, the median APPQ was 0.14.

Item Type:Article
Status:Published
Refereed:Yes
Glasgow Author(s):Preston, Prof Thomas and McMillan, Prof Donald and Slater, Dr Christine
Authors: McMillan, D.C., Slater, C., Preston, T., Falconer, J.S., and Fearon, K.C.H.
College/School:College of Medical Veterinary and Life Sciences > School of Medicine
College of Medical Veterinary and Life Sciences > School of Medicine > Clinical Specialities
College of Science and Engineering > Scottish Universities Environmental Research Centre
Journal Name:Nutrition
ISSN:0899-9007
Published Online:25 March 1999

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