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Protein synthesis in cancer patients with inflammatory response: investigations with [15N]glycine

McMillan, D.C., Preston, T., Fearon, K.C., Burns, H.J., Slater, C., and Shenkin, A. (1994) Protein synthesis in cancer patients with inflammatory response: investigations with [15N]glycine. Nutrition, 10 (3). pp. 232-240. ISSN 0899-9007

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Abstract

It has been proposed that the increase in amino acid flux and derived protein synthesis rates observed in weight-losing cancer patients may contribute to an ongoing negative energy balance. The mediators and tissues responsible for such apparent increased protein synthesis have not been clearly identified. The aim of this study was to examine the relationship between protein synthetic rates in whole-body, skeletal muscle, and circulating cortisol concentrations in healthy subjects (n = 6) and cancer patients with evidence of an inflammatory response (n = 6). Protein synthetic rates were measured with a primed continuous 20-h infusion of [15N]glycine. Skeletal muscle was biopsied at laparotomy. Serum cortisol, resting energy expenditure, plasma proteins, nitrogen metabolites in urine, and skeletal muscle free amino acids were also measured. Derived whole-body and skeletal muscle protein synthetic rates in the cancer group were increased significantly (by 70 and 93%, respectively, p < 0.05). Circulating concentrations of cortisol, fibrinogen, and C-reactive protein were also significantly increased in the cancer group and indicated the presence of an inflammatory response. However, there was no significant increase in resting energy expenditure. Mechanisms by which apparent increases in whole-body and skeletal protein synthesis do not result in an increase in resting energy expenditure are discussed. We conclude that glycine utilization is increased in cancer patients but that rates of protein synthesis derived from [15N]glycine kinetics may not be valid in such patients.

Item Type:Article
Status:Published
Refereed:Yes
Glasgow Author(s):Preston, Prof Thomas and McMillan, Prof Donald
Authors: McMillan, D.C., Preston, T., Fearon, K.C., Burns, H.J., Slater, C., and Shenkin, A.
College/School:College of Medical Veterinary and Life Sciences > School of Medicine > Clinical Specialities
College of Science and Engineering > Scottish Universities Environmental Research Centre
Journal Name:Nutrition
ISSN:0899-9007
ISSN (Online):1873-1244
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