In ovo non-invasive quantification of the myocardial function and mass of chick embryos using magnetic resonance imaging

Holmes, W.H. , McCabe, C. , Mullin, J.M., Condon, B. and Bain, M.M. (2009) In ovo non-invasive quantification of the myocardial function and mass of chick embryos using magnetic resonance imaging. NMR in Biomedicine, 22(7), pp. 745-752. (doi: 10.1002/nbm.1386)

Abstract

Magnetic resonance imaging (MRI) has evolved as one of the major non-invasive tools to study healthy and diseased hearts in animal models, especially rodent models. Even though, the chick embryo has long been used as a model for cardiovascular research, MRI has not yet been used for <i>in vivo</i> cardiac studies. Part of the reason for this is the difficulty in monitoring the ECG and respiration of the chick embryo in the magnet for gating purposes. To overcome this complication, this paper presents the use of retrospective Cine MRI to measure the cardiac function of chick embryos <i>in ovo</i> for the first time, without the need for respiratory or cardiac gating. The resulting left ventricular functional parameters, from six chick embryos at 20 days of incubation, were (meanWSD) EDV 69±15μL, ESV 31±7μL, SV 38±9μL and EF 54.5±2%. The use of retrospective Cine MRI at earlier stages of development is also discussed and difficulties have been highlighted.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Condon, Professor Barrie and Mullin, Mr James and Holmes, Dr William and Bain, Professor Maureen and McCabe, Dr Chris
• Authors: Holmes, W.H., McCabe, C., Mullin, J.M., Condon, B., and Bain, M.M.
• College/School: College of Medical Veterinary and Life Sciences > School of Psychology & Neuroscience; College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Clinical Specialities; College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
• Research Group: Glasgow Experimental MRI Centre
• Journal Name: NMR in Biomedicine
• ISSN: 0952-3480
• ISSN (Online): 1099-1492
• Published Online: 25 March 2009