The development of RNA interference (RNAi) in gastrointestinal nematodes

Selkirk, M.E., Huang, S.C., Knox, D.P. and Britton, C. (2012) The development of RNA interference (RNAi) in gastrointestinal nematodes. Parasitology, 139(5), pp. 605-612. (doi:10.1017/S0031182011002332)

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Abstract

Despite the utility of RNAi for defining gene function in Caenorhabditis elegans and early successes reported in parasitic nematodes, RNAi has proven to be stubbornly inconsistent or ineffective in the animal parasitic nematodes examined to date. Here, we summarise some of our experiences with RNAi in parasitic nematodes affecting animals and discuss the available data in the context of our own unpublished work, taking account of mode of delivery, larval activation, site of gene transcription and the presence/absence of essential RNAi pathway genes as defined by comparisons to C. elegans. We discuss future directions briefly including the evaluation of nanoparticles as a means to enhance delivery of interfering RNA to the target worm tissue.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Britton, Dr Collette
Authors: Selkirk, M.E., Huang, S.C., Knox, D.P., and Britton, C.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Parasitology
ISSN:0031-1820
Published Online:01 March 2012

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