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Birds are difficult to sex. Nestlings rarely show sex-linked morphology and we estimate that adult females appear identical to males in over 50% of the world's bird species. This problem can hinder both evolutionary studies and human-assisted breeding of birds. DNA-based sex identification provides a solution. We describe a test based on two conserved CHD (chromo-helicase-DNA-binding) genes that are located on the avian sex chromosomes of all birds, with the possible exception of the ratites (ostriches, etc.; Struthioniformes). The CHD-W gene is located on the W chromosome; therefore it is unique to females. The other gene, CHD-Z, is found on the Z chromosome and therefore occurs in both sexes (female, ZW; male, ZZ). The test employs PCR with a single set of primers. It amplifies homologous sections of both genes and incorporates introns whose lengths usually differ. When examined on a gel there is a single CHD-Z band in males but females have a second, distinctive CHD-W band.
|Glasgow Author(s) Enlighten ID:||Griffiths, Mrs Katharine and Griffiths, Dr Richard|
|Authors:||Griffiths, R., Double, M.C., Orr, K., and Dawson, R.J.G.|
|College/School:||College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine|
College of Medical Veterinary and Life Sciences > School of Life Sciences
|Journal Name:||Molecular Ecology|
|Published Online:||28 February 2002|