Griffiths, R. and Orr, K. (1999) The use of amplified fragment length polymorphism (AFLP) in the isolation of sex-specific markers. Molecular Ecology, 8(4), pp. 671-674. (doi: 10.1046/j.1365-294x.1999.00578.x)
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Abstract
Sex identification is a problem in research and conservation. It can often be solved using a DNA test but this is only an option if a sex-specific marker is available. Such markers can be identified using the amplified fragment length polymorphism (AFLP) technique. This is usually a taxonomic method, as it produces a DNA fingerprint of 50–100 PCR bands. However, if male and female AFLP products are compared, sex-specific markers are confined to the heterogametic sex and can rapidly be identified. Once a marker is found, AFLP can be used to sex organisms directly or the marker can be sequenced and a standard PCR test designed.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Griffiths, Mrs Kate and Griffiths, Dr Richard |
| Authors: | Griffiths, R., and Orr, K. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Life Sciences College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
| Journal Name: | Molecular Ecology |
| ISSN: | 0962-1083 |
| ISSN (Online): | 1365-294X |
| Published Online: | 15 December 2003 |
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