Griffiths, R., and Orr, K. (1999) The use of amplified fragment length polymorphism (AFLP) in the isolation of sex-specific markers. Molecular Ecology, 8 (4). pp. 671-674. ISSN 0962-1083 (doi:10.1046/j.1365-294x.1999.00578.x)
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Sex identification is a problem in research and conservation. It can often be solved using a DNA test but this is only an option if a sex-specific marker is available. Such markers can be identified using the amplified fragment length polymorphism (AFLP) technique. This is usually a taxonomic method, as it produces a DNA fingerprint of 50–100 PCR bands. However, if male and female AFLP products are compared, sex-specific markers are confined to the heterogametic sex and can rapidly be identified. Once a marker is found, AFLP can be used to sex organisms directly or the marker can be sequenced and a standard PCR test designed.
|Glasgow Author(s):||Griffiths, Mrs Katharine and Griffiths, Dr Richard|
|Authors:||Griffiths, R., and Orr, K.|
|College/School:||College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine|
College of Medical Veterinary and Life Sciences > School of Life Sciences
|Journal Name:||Molecular Ecology|
|Published Online:||15 December 2003|