Enlighten
Research publications by members of the University of Glasgow
home > services > Enlighten

The use of amplified fragment length polymorphism (AFLP) in the isolation of sex-specific markers

Griffiths, R., and Orr, K. (1999) The use of amplified fragment length polymorphism (AFLP) in the isolation of sex-specific markers. Molecular Ecology, 8 (4). pp. 671-674. ISSN 0962-1083 (doi:10.1046/j.1365-294x.1999.00578.x)

Full text not currently available from Enlighten.

Abstract

Sex identification is a problem in research and conservation. It can often be solved using a DNA test but this is only an option if a sex-specific marker is available. Such markers can be identified using the amplified fragment length polymorphism (AFLP) technique. This is usually a taxonomic method, as it produces a DNA fingerprint of 50–100 PCR bands. However, if male and female AFLP products are compared, sex-specific markers are confined to the heterogametic sex and can rapidly be identified. Once a marker is found, AFLP can be used to sex organisms directly or the marker can be sequenced and a standard PCR test designed.

Item Type:Article
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Griffiths, Mrs Katharine and Griffiths, Dr Richard
Authors: Griffiths, R., and Orr, K.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:Molecular Ecology
ISSN:0962-1083
ISSN (Online):1365-294X
Published Online:15 December 2003

University Staff: Request a correction | Enlighten Editors: Update this record