Trypanosoma vivax displays a clonal population structure

Duffy, C. W., Morrison, L. J., Black, A., Pinchbeck, G. L., Christley, R. M., Schoenefeld, A., Tait, A., Turner, C.M. and MacLeod, A. (2009) Trypanosoma vivax displays a clonal population structure. International Journal for Parasitology, 39(13), 1475 -1483. (doi:10.1016/j.ijpara.2009.05.012) (PMID:19520081)

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Abstract

African animal trypanosomiasis, or Nagana, is a debilitating and economically costly disease with a major impact on animal health in sub-Saharan Africa. Trypanosoma vivax, one of the principal trypanosome species responsible for the disease, infects a wide host range including cattle, goats, horses and donkeys and is transmitted both cyclically by tsetse flies and mechanically by other biting flies, resulting in a distribution covering large swathes of South America and much of sub-Saharan Africa. While there is evidence for mating in some of the related trypanosome species, Trypanosoma brucei, Trypanosoma congolense and Trypanosoma cruzi, very little work has been carried out to examine this question in T. vivax. Understanding whether mating occurs in T. vivax will provide insight into the dynamics of trait inheritance, for example the spread of drug resistance, as well as examining the origins of meiosis in the order Kinetoplastida. With this in mind we have identified orthologues of eight core meiotic genes within the genome, the presence of which imply that the potential for mating exists in this species. In order to address whether mating occurs, we have investigated a sympatric field population of T. vivax collected from livestock in The Gambia, using microsatellite markers developed for this species. Our analysis has identified a clonal population structure showing significant linkage disequilibrium, homozygote deficits and disagreement with Hardy–Weinberg predictions at six microsatellite loci, indicative of a lack of mating in this population of T. vivax.

Item Type:Articles
Keywords:<i>Trypanosoma vivax</i>, Population genetics, Mating, Microsatellites, Multilocus genotypes
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:MacLeod, Professor Annette and Tait, Professor Andy and Morrison, Dr Liam and Turner, Professor Charles
Authors: Duffy, C. W., Morrison, L. J., Black, A., Pinchbeck, G. L., Christley, R. M., Schoenefeld, A., Tait, A., Turner, C.M., and MacLeod, A.
Subjects:Q Science > QL Zoology
Q Science > QH Natural history > QH426 Genetics
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:International Journal for Parasitology
Publisher:Elsevier
ISSN:0020-7519
ISSN (Online):1879-0135
Published Online:08 June 2009

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