Interaction specificity of Arabidopsis 14-3-3 proteins with phototropin receptor kinases

Sullivan, S. , Thompson, C. E., Kaiserli, E. and Christie, J. M. (2009) Interaction specificity of Arabidopsis 14-3-3 proteins with phototropin receptor kinases. FEBS Letters, 583(13), pp. 2187-2193. (doi: 10.1016/j.febslet.2009.06.011) (PMID:19524572)

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Publisher's URL: http://dx.doi.org/10.1016/j.febslet.2009.06.011

Abstract

Phototropin receptor kinases play an important roles in optimising plant growth in response to blue light. Much is known regarding their photochemical reactivity, yet little progress has been made to identify downstream signalling components. Here, we isolated several interacting proteins for Arabidopsis phototropin 1 (phot1) by yeast two-hybrid screening. These include members of the NPH3/RPT2 (NRL) protein family, proteins associated with vesicle trafficking, and the 14-3-3 lambda (?) isoform from Arabidopsis . 14-3-3? and phot1 were found to colocalise and interact in vivo. Moreover, 14-3-3 binding to phot1 was limited to non-epsilon 14-3-3 isoforms and was dependent on key sites of receptor autophosphorylation. No 14-3-3 binding was detected for Arabidopsis phot2, suggesting that 14-3-3 proteins represent specific mode of phot1 signalling.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Sullivan, Dr Stuart and Kaiserli, Dr Eirini and Christie, Professor John
Authors: Sullivan, S., Thompson, C. E., Kaiserli, E., and Christie, J. M.
Subjects:Q Science > QH Natural history > QH301 Biology
Q Science > QK Botany
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:FEBS Letters
Publisher:Elsevier
ISSN:0014-5793
ISSN (Online):1873-3468
Published Online:12 June 2009
Copyright Holders:Copyright © 2009 Federation of European Biochemical Societies
First Published:First published in Febs Letters 583(13): 2187-2193
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
378561Characterisation of the phot1-interacting protein AtMRD1 and its role in regulating phototropismJohn ChristieBiotechnology and Biological Sciences Research Council (BBSRC)BB/C000366/1Institute of Molecular Cell and Systems Biology