Jecna, L., Svarovska, A., Besteiro, S., Mottram, J. C. , Coombs, G. H. and Volf, P. (2009) Inhibitor of cysteine peptidase does not influence the development of Leishmania mexicana in Lutzomyia longipalpis. Journal of Medical Entomology, 46(3), pp. 605-609. (doi: 10.1603/033.046.0327)
Full text not currently available from Enlighten.
Publisher's URL: http://dx.doi.org/10.1603/033.046.0327
Abstract
It has been proposed that the natural cysteine peptidase inhibitor ICP of Leishmania mexicana protects the protozoan parasite from insect host proteolytic enzymes, thereby promoting, survival. To test this hypothesis L. mexicana mutants deficient in ICP were evaluated for their ability to develop in the sand fly Lutzomyia longipalpis. No significant differences were found between the wild-type parasites, two independently derived ICP-deficient mutants, or mutants overexpressing ICP all lines developed similarly in the sand fly midgut and produced heavy late-stage infections. In addition, recombinant L. mexicana ICP did not inhibit peptidase activity of the midgut extracts in vitro. We conclude that ICP has no major role in promoting survival of L. mexicana in the vectorial part of its life cycle in L. longipalpis
| Item Type: | Articles |
|---|---|
| Keywords: | Sand fly, Leishmania, Phlebotomus, cysteine peptidase |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Coombs, Professor Graham and Mottram, Professor Jeremy |
| Authors: | Jecna, L., Svarovska, A., Besteiro, S., Mottram, J. C., Coombs, G. H., and Volf, P. |
| Subjects: | Q Science > QR Microbiology > QR355 Virology Q Science > QH Natural history > QH345 Biochemistry |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Journal of Medical Entomology |
| Publisher: | Entomological Society of America - |
| ISSN: | 0022-2585 |
| ISSN (Online): | 1938-2928 |
| Related URLs: |
University Staff: Request a correction | Enlighten Editors: Update this record
Funder and Project Information
Funder and Project Information