Characterization of two distinct binding modes between syntaxin 4 and Munc18c

Aran-Ponte, V., Brandie, F.M., Boyd, A.R., Kantidakis, T., Rideout, E.J., Kelly, S.M. , Gould, G.W. and Bryant, N.J. (2009) Characterization of two distinct binding modes between syntaxin 4 and Munc18c. Biochemical Journal, 419(Pt3), pp. 655-660. (doi:10.1042/BJ20082293)

Full text not currently available from Enlighten.

Abstract

Interaction of SM (Sec1/Munc18) proteins with their cognate syntaxins represents an important regulatory mechanism of SNARE (soluble N-ethylmaleimide-sensitive fusion protein-attachment protein receptor)-mediated membrane fusion. Understanding the conserved mechanisms by which SM proteins function in this process has proved challenging, largely due to an apparent lack of conservation of binding mechanisms between different SM-syntaxin pairs. In the present study, we have identified a hitherto uncharacterized mode of binding between syntaxin 4 and Munc18c that is independent of the binding mode shown previously to utilize the N-terminal peptide of syntaxin 4. Our data demonstrate that syntaxin 4 and Munc18c interact via two distinct modes of binding, analogous to those employed by syntaxin 1a-Munc18a and syntaxin 16-Vps45p (vacuolar protein sorting 45). These data support the notion that all syntaxin/SM proteins bind using conserved mechanisms, and pave the way for the formulation of unifying hypotheses of SM protein function

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Brandie, Ms Fiona and Gould, Professor Gwyn and Bryant, Dr Nia and Kantidakis, Dr Theodoros and Kelly, Dr Sharon
Authors: Aran-Ponte, V., Brandie, F.M., Boyd, A.R., Kantidakis, T., Rideout, E.J., Kelly, S.M., Gould, G.W., and Bryant, N.J.
Subjects:Q Science > QH Natural history > QH345 Biochemistry
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Biochemical Journal
Journal Abbr.:Biochem. J.
Publisher:Portland Press Ltd.
ISSN:0264-6021
ISSN (Online):1470-8728
Published Online:04 February 2009

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
404401Functional analysis of Munc18c/Syntaxin4 interactionsNia BryantBiotechnology and Biological Sciences Research Council (BBSRC)BB/D000211/1Institute of Molecular Cell and Systems Biology