A prospective study in children with a severe form of atopic dermatitis: clinical outcome in relation to cytokine gene polymorphisms

Kayserova, J., Sismova, S., Zentsova-Jaresova, I., Katina, S., Vernerova, E., Polouckova, A., Malinova, V., Striz, I. and Sediva, A. (2012) A prospective study in children with a severe form of atopic dermatitis: clinical outcome in relation to cytokine gene polymorphisms. Journal of Investigational Allergology and Clinical Immunology, 22(2), pp. 92-101.

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Publisher's URL: http://www.jiaci.org/issues/vol22issue2/vol22issue02-3.htm

Abstract

Background and Objective: <br/> <br/> The course of atopic dermatitis (AD) in childhood is characterized by typical changes in phenotype, including a shift from skin involvement to respiratory allergy usually around the third year of age. We thus designed a prospective study to monitor the outcome of severe AD and to investigate the association between cytokine gene polymorphisms and clinical manifestations. Methods: <br/> <br/> Clinical and laboratory follow-up of 94 patients with severe AD and 103 healthy controls was performed using routine methodology. Allele, genotype, and haplotype frequencies of single nucleotide polymorphisms of 13 selected cytokine/receptor genes were analyzed using PCR with sequence-specifi c primers. Results: <br/> <br/> In our study, genotypes of 7 polymorphisms—IL-4 -1098G/T and -590C/T, IL-6 -174C/G and nt565A/G, and IL-10 -1082A/G, -819C/T, and -592A/C were signifi cantly associated with atopic AD (P<.05). A signifi cant association was also found for TNF-α AA and IL-4 GC haplotypes and AD. We confirm the progressive clinical improvement of AD together with a decrease in the severity index SCORAD (SCORing atopic dermatitis) during childhood (P<.05). We found signifi cant differences between IL-4Rα +1902 A/G and positivity of tree pollen–specifi c IgE (P<.05) in the AD group. Moreover, a weak association was also found between IL-10 -819C/T and IL-10 -590A/C and the appearance of allergic rhinitis (P<0.1). Conclusions: <br/> <br/> We confirmed a clinical shift in allergic phenotype in the fi rst 3 years of life, and showed an association between IL-4, IL-6, and IL-10 polymorphisms and AD. Our data indicate that IL-4α and IL-10 polymorphisms may be considered predictive factors of respiratory allergy in children with AD.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Katina, Dr Stanislav
Authors: Kayserova, J., Sismova, S., Zentsova-Jaresova, I., Katina, S., Vernerova, E., Polouckova, A., Malinova, V., Striz, I., and Sediva, A.
Subjects:Q Science > QA Mathematics
Q Science > QR Microbiology > QR180 Immunology
College/School:College of Science and Engineering > School of Mathematics and Statistics > Statistics
Research Group:Statistical Modelling
Journal Name:Journal of Investigational Allergology and Clinical Immunology
Journal Abbr.:J Investig Allergol Clin Immunol
Publisher:Esmon Publicidad
ISSN:1018-9068
ISSN (Online):1698-0808
Published Online:01 January 2012

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