Noninvasive MRI measurement of CBF: evaluating an arterial spin labelling sequence with 99mTc- HMPAO CBF autoradiography in a rat stroke model

Baskerville, T., McCabe, C. , Weir, C.J., Macrae, I.M. and Holmes, W. (2012) Noninvasive MRI measurement of CBF: evaluating an arterial spin labelling sequence with 99mTc- HMPAO CBF autoradiography in a rat stroke model. Journal of Cerebral Blood Flow and Metabolism, 32(6), pp. 973-977. (doi:10.1038/jcbfm.2012.19) (PMID:22472604) (PMCID:PMC3367221)

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Abstract

Arterial spin labelling (ASL) is increasingly available for noninvasive cerebral blood flow (CBF) measurement in stroke research. Here, a pseudo-continuous ASL technique (pCASL) was evaluated against 99mTc-D, L-hexamethylpropyleneamine oxime (99mTc-HMPAO) autoradiography in a rat stroke model. The 99mTc-HMPAO was injected (intravenously, 225MBq) during pCASL acquisition. The pCASL and 99mTc-HMPAO autoradiography CBF measures, relative to the contralateral hemisphere, were in good agreement across the spectrum of flow values in normal and ischemic tissues. The pCASL-derived quantitative regional CBF values (contralateral: 157 to 177 mL/100 g per minute; ipsilateral: 9 to 104 mL/100 g per minute) were consistent with the literature values. The data show the potential utility of pCASL for CBF assessment in a rat stroke model.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Macrae, Professor I Mhairi and Holmes, Dr William and McCabe, Dr Chris and Baskerville, Dr Tracey
Authors: Baskerville, T., McCabe, C., Weir, C.J., Macrae, I.M., and Holmes, W.
Subjects:Q Science > Q Science (General)
College/School:College of Medical Veterinary and Life Sciences > Institute of Neuroscience and Psychology
Research Group:Glasgow Experimental MRI Centre
Journal Name:Journal of Cerebral Blood Flow and Metabolism
Publisher:Nature Publishing Group
ISSN:0271-678X
ISSN (Online):1559-7016
Published Online:04 April 2012

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
455001Imaging the ischaemic penumbra using BOLD MRI with oxygen challenge as a biotracerI Mhairi MacraeMedical Research Council (MRC)G0700439RI NEUROSCIENCE & PSYCHOLOGY