Sandvig, I. et al. (2012) In vivo MRI of olfactory ensheathing cell grafts and regenerating axons in transplant mediated repair of the adult rat optic nerve. NMR in Biomedicine, 25(4), pp. 620-631. (doi: 10.1002/nbm.1778)
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Abstract
The purpose of the present study was to use magnetic resonance imaging (MRI) as a tool for monitoring transplant-mediated repair of the adult rat visual pathway. We labelled rat olfactory ensheathing cells (OECs) using micron-sized particles of iron oxide (MPIO) and transplanted them by: i) intravitreal injection (ivit) and ii) intra-optic nerve (ON) injection (iON) in adult rats with ON crush (ONC) injury. We applied T2-weighted MRI and manganese-enhanced MRI (MEMRI) to visualise transplanted cells and ON axons at specific times after injury and cell engraftment. Our findings demonstrate that ivit MPIO-labelled OECs are unequivocally detected by T2-weighted MRI in vivo and that the T1-weighted 3D FLASH sequence applied for MEMRI facilitates simultaneous visualisation of Mn2+−enhanced regenerating retinal ganglion cell (RGC) axons and MPIO-labelled OEC grafts. Furthermore, analysis of MRI data and ultrastructural findings supports the hypothesis that iON OEC transplants mediate regeneration and remyelination of RGC axons post injury.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Barnett, Professor Susan and Sardella, Dr Thomas |
Authors: | Sandvig, I., Thuen, M., Hoang, L., Olsen, Ø., Sardella, T.C.P., Brekken, C., Tvedt, K.E., Barnett, S.C., Haraldseth, O., Berry, M., and Sandvig, A. |
College/School: | College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation College of Medical Veterinary and Life Sciences > School of Psychology & Neuroscience |
Journal Name: | NMR in Biomedicine |
ISSN: | 0952-3480 |
Published Online: | 26 August 2011 |
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