Alternative splicing mediates responses of the arabidopsis circadian clock to temperature changes

James, A.B., Syed, N.H., Bordage, S., Marshall, J., Nimmo, G.M., Jenkins, G.I. , Herzyk, P. , Brown, J.W.S. and Nimmo, H.G. (2012) Alternative splicing mediates responses of the arabidopsis circadian clock to temperature changes. Plant Cell, 24(3), pp. 961-981. (doi:10.1105/tpc.111.093948) (PMID:22408072) (PMCID:PMC3336117)

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Abstract

Alternative splicing plays crucial roles by influencing the diversity of the transcriptome and proteome and regulating protein structure/function and gene expression. It is widespread in plants, and alteration of the levels of splicing factors leads to a wide variety of growth and developmental phenotypes. The circadian clock is a complex piece of cellular machinery that can regulate physiology and behavior to anticipate predictable environmental changes on a revolving planet. We have performed a system-wide analysis of alternative splicing in clock components in Arabidopsis thaliana plants acclimated to different steady state temperatures or undergoing temperature transitions. This revealed extensive alternative splicing in clock genes and dynamic changes in alternatively spliced transcripts. Several of these changes, notably those affecting the circadian clock genes LATE ELONGATED HYPOCOTYL (LHY) and PSEUDO RESPONSE REGULATOR7, are temperature-dependent and contribute markedly to functionally important changes in clock gene expression in temperature transitions by producing nonfunctional transcripts and/or inducing nonsense-mediated decay. Temperature effects on alternative splicing contribute to a decline in LHY transcript abundance on cooling, but LHY promoter strength is not affected. We propose that temperature-associated alternative splicing is an additional mechanism involved in the operation and regulation of the plant circadian clock.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Nimmo, Dr Gillian and Nimmo, Professor Hugh and Herzyk, Dr Pawel and Jenkins, Professor Gareth and James, Dr Allan
Authors: James, A.B., Syed, N.H., Bordage, S., Marshall, J., Nimmo, G.M., Jenkins, G.I., Herzyk, P., Brown, J.W.S., and Nimmo, H.G.
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Plant Cell
Publisher:American Society of Plant Biologists
ISSN:1040-4651
ISSN (Online):1532-298X
Published Online:09 March 2012
Copyright Holders:Copyright © 2012 American Society of Plant Biologists
First Published:First published in Plant Cell 24(3):961-981
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
470041Organ specificity and communication in the plant circadian clockHugh NimmoLeverhulme Trust (LEVERHULME)RF/2/RFG/2008/0RI MOLECULAR CELL & SYSTEMS BIOLOGY
480671Organ communication in the Arabidopsis circadian clockHugh NimmoBiotechnology and Biological Sciences Research Council (BBSRC)BB/G008752/1RI MOLECULAR CELL & SYSTEMS BIOLOGY