Martino, C., Horsfall, L., Chen, Y., Chanasakulniyom, M., Paterson, D., Brunet, A., Rosser, S., Yuan, Y.-J. and Cooper, J.M. (2012) Cytoskeletal protein expression and its association within the hydrophobic membrane of artificial cell models. ChemBioChem, 13(6), pp. 792-795. (doi: 10.1002/cbic.201200038)
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Publisher's URL: http://dx.doi.org/10.1002/cbic.201200038
Abstract
We demonstrate the cell-free expression of the actin-like protein MreB and observed its preference to localize and aggregate at the membrane interface of a water-in-oil-in-water droplet as happens in vivo. These monodisperse microdroplets were produced on a large scale using microfluidics. We suggest a possible use of this platform for theory validation in fundamental biological studies.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Rosser, Professor Susan and Horsfall, Dr Louise and Cooper, Professor Jonathan |
Authors: | Martino, C., Horsfall, L., Chen, Y., Chanasakulniyom, M., Paterson, D., Brunet, A., Rosser, S., Yuan, Y.-J., and Cooper, J.M. |
College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
Journal Name: | ChemBioChem |
ISSN: | 1439-4227 |
ISSN (Online): | 1439-7633 |
Published Online: | 12 March 2012 |
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