Cytoskeletal protein expression and its association within the hydrophobic membrane of artificial cell models

Martino, C., Horsfall, L., Chen, Y., Chanasakulniyom, M., Paterson, D., Brunet, A., Rosser, S., Yuan, Y.-J. and Cooper, J.M. (2012) Cytoskeletal protein expression and its association within the hydrophobic membrane of artificial cell models. ChemBioChem, 13(6), pp. 792-795. (doi:10.1002/cbic.201200038)

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Publisher's URL: http://dx.doi.org/10.1002/cbic.201200038

Abstract

We demonstrate the cell-free expression of the actin-like protein MreB and observed its preference to localize and aggregate at the membrane interface of a water-in-oil-in-water droplet as happens in vivo. These monodisperse microdroplets were produced on a large scale using microfluidics. We suggest a possible use of this platform for theory validation in fundamental biological studies.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Rosser, Professor Susan and Horsfall, Dr Louise and Cooper, Professor Jonathan
Authors: Martino, C., Horsfall, L., Chen, Y., Chanasakulniyom, M., Paterson, D., Brunet, A., Rosser, S., Yuan, Y.-J., and Cooper, J.M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:ChemBioChem
ISSN:1439-4227
ISSN (Online):1439-7633
Published Online:12 March 2012

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