Evaluation of coupling reversed phase, aqueous normal phase, and hydrophilic interaction liquid chromatography with orbitrap mass spectrometry for metabolomic studies of human urine

Zhang, T., Creek, D.J., Barrett, M.P. , Blackburn, G. and Watson, D.G. (2012) Evaluation of coupling reversed phase, aqueous normal phase, and hydrophilic interaction liquid chromatography with orbitrap mass spectrometry for metabolomic studies of human urine. Analytical Chemistry, 84(4), pp. 1994-2001. (doi:10.1021/ac2030738)

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Abstract

In this study, we assessed three liquid chromatographic platforms: reversed phase (RP), aqueous normal phase (ANP), and hydrophilic interaction (HILIC) for the analysis of polar metabolite standard mixtures and for their coverage of urinary metabolites. The two zwitterionic HILIC columns showed high-quality chromatographic performance for metabolite standards, improved separation for isomers, and the greatest coverage of polar metabolites in urine. In contrast, on the reversed phase column, most metabolites eluted very rapidly with little or no separation. Using an Exactive Orbitrap mass spectrometer with a HILIC liquid chromatographic platform, approximately 970 metabolite signals with repeatable peak areas (relative standard deviation (RSD) ≤ 25%) could be putatively identified in human urine, by elemental composition assignment within a 3 ppm mass error. The ability of the methodology for the verification of nonmolecular ions, which arise from adduct formation, and the possibility of distinguishing isomers could also be demonstrated. Careful examination of the raw data and the use of masses for predicted metabolites produced an extension of the metabolite list for human urine.

Item Type:Articles
Additional Information:This document is the unedited Author’s version of a Submitted Work that was subsequently accepted for publication in Analytical Chemistry, copyright © American Chemical Society after peer review. To access the final edited and published work see http://dx.doi.org/10.1021/ac2030738
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Barrett, Professor Michael and Creek, Dr Darren
Authors: Zhang, T., Creek, D.J., Barrett, M.P., Blackburn, G., and Watson, D.G.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:Analytical Chemistry
Journal Abbr.:Anal. chem.
Publisher:American Chemical Society
ISSN:0003-2700
ISSN (Online):1520-6882
Published Online:18 January 2012
Copyright Holders:Copyright © 2012 American Chemical Society
First Published:First published in Analytical Chemistry 84(4):1994-2001
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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