Gibson, V.B. et al. (2012) A novel method to allow noninvasive, longitudinal imaging of the murine immune system in vivo. Blood, 119(11), pp. 2545-2551. (doi: 10.1182/blood-2011-09-378356) (PMID:22271449) (PMCID:PMC3398133)
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Abstract
In vivo imaging has revolutionized understanding of the spatiotemporal complexity that subserves the generation of successful effector and regulatory immune responses. Until now, invasive surgery has been required for microscopic access to lymph nodes (LNs), making repeated imaging of the same animal impractical and potentially affecting lymphocyte behavior. To allow longitudinal in vivo imaging, we conceived the novel approach of transplanting LNs into the mouse ear pinna. Transplanted LNs maintain the structural and cellular organization of conventional secondary lymphoid organs. They participate in lymphocyte recirculation and exhibit the capacity to receive and respond to local antigenic challenge. The same LN could be repeatedly imaged through time without the requirement for surgical exposure, and the dynamic behavior of the cells within the transplanted LN could be characterized. Crucially, the use of blood vessels as fiducial markers also allowed precise re-registration of the same regions for longitudinal imaging. Thus, we provide the first demonstration of a method for repeated, noninvasive, in vivo imaging of lymphocyte behavior.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | McInnes, Professor Iain and Rush, Dr Catherine and Garside, Professor Paul and Brewer, Professor James and Benson, Dr Robert and Maffia, Professor Pasquale and Grassia, Dr Gianluca and Bryson, Dr Karen |
| Authors: | Gibson, V.B., Benson, R.A., Bryson, K.J., McInnes, I.B., Rush, C.M., Grassia, G., Maffia, P., Jenkinson, E.J., White, A.J., Anderson, G., Brewer, J.M., and Garside, P. |
| Subjects: | Q Science > QR Microbiology > QR180 Immunology |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Blood |
| Publisher: | American Society of Hematology |
| ISSN: | 0006-4971 |
| ISSN (Online): | 1528-0020 |
| Published Online: | 23 January 2012 |
| Copyright Holders: | Copyright © 2012 American Society of Hematology |
| First Published: | First published in Blood 119(11):2545-2551 |
| Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher |
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