Inhibitory effects of PPAR-γ on endothelin-1-induced inflammatory pathways in vascular smooth muscle cells from normotensive and hypertensive rats

Montezano, A.C., Amiri, F., Tostes, R.C., Touyz, R.M. and Schiffrin, E.L. (2007) Inhibitory effects of PPAR-γ on endothelin-1-induced inflammatory pathways in vascular smooth muscle cells from normotensive and hypertensive rats. Journal of the American Society of Hypertension, 1(2), pp. 150-160. (doi:10.1016/j.jash.2007.01.005)

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Publisher's URL: http://dx.doi.org/10.1016/j.jash.2007.01.005

Abstract

The present study evaluated the effects of endothelin (ET)-1 and the peroxisome proliferator activated receptor γ (PPAR-γ) agonist, rosiglitazone, on inflammatory markers in vascular smooth muscle cells (VSMCs) from normotensive (WKY) and hypertensive (SHRSP) rats. Rat VSMC-derived mesenteric arteries from WKY and SHRSP were treated with ET-1 (100 mmol/L) and rosiglitazone (1μmol/L) or ET type A (ETA) or type B (ETB) receptor antagonists. Nuclear factor kappa-B (NFκB) binding activity was assessed by electrophoretic mobility shift assay and phospho-inhibitory κB (IκB); vascular cell adhesion molecule (VCAM)-1, intercellular adhesion molecule (ICAM)-1, and cyclooxygenase (COX)-2 expression was determined using Western blotting. ET-1 significantly increased NFκB binding, and VCAM-1, ICAM, and COX-2 expression to a greater degree in SHRSP than in WKY VSMC. These changes were associated with increased phosphorylation of IκB, thus resulting in decreased NFκB inhibition. Co-incubation with PPAR-γ activator rosiglitazone, or ETA or ETB receptor antagonism prevented ET-1-stimulated vascular proinflammatory effects in both WKY and SHRSP VSMC. Proinflammatory effects of ET-1 in VSMCs are mediated via both ETA and ETB receptor subtypes. These effects may be abrogated by the PPAR-γ activator rosiglitazone. PPAR-γ activators may thus prevent deleterious ET-1-dependent proinflammatory vascular effects in VSMC in hypertension.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Montezano, Dr Augusto and Touyz, Professor Rhian
Authors: Montezano, A.C., Amiri, F., Tostes, R.C., Touyz, R.M., and Schiffrin, E.L.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
Journal Name:Journal of the American Society of Hypertension
ISSN:1933-1711
ISSN (Online):1878-7436
Published Online:27 March 2007

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