Directed antigen targeting in vivo identifies a role for CD103+ dendritic cells in both tolerogenic and immunogenic T-cell responses

Semmrich, M., Plantinga, M., Svensson-Frej, M., Uronen-Hansson, H., Gustafsson, T., Mowat, A.M., Yrlid, U., Lambrecht, B.N. and Agace, W.W. (2012) Directed antigen targeting in vivo identifies a role for CD103+ dendritic cells in both tolerogenic and immunogenic T-cell responses. Mucosal Immunology, 5(2), pp. 150-160. (doi: 10.1038/mi.2011.61)

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Publisher's URL: http://dx.doi.org/10.1038/mi.2011.61

Abstract

The αE integrin chain CD103 identifies a subset of migratory dendritic cells (DCs) in the gut, lung, and skin. To gain further understanding of the function of CD103+ DCs in regulating adaptive immunity in vivo, we coupled ovalbumin (OVA) to the CD103 antibody M290 (M290.OVA). Intraperitoneal injection of M290.OVA induced OVA-specific CD8+ and CD4+ T-cell proliferation in lymph nodes (LNs) of wild-type but not CD103−/− mice, or in mice depleted of CD11c+ cells. In the absence of maturation stimuli, systemic antigen targeting to CD103+ DCs led to tolerance of CD8+ T cells, whereas coadministration of adjuvant induced cytotoxic T-lymphocyte (CTL) immunity and antibody production. Mucosal intratracheal application of M290.OVA also induced T-cell proliferation in mediastinal LNs, yet the functional outcome was tolerance that inhibited subsequent development of allergic airway inflammation and immunoglobulin E (IgE) responses to inhaled OVA. These findings identify antigen targeting to CD103+ DCs as a potential strategy to regulate immune responses in nonlymphoid mucosal tis

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Mowat, Professor Allan and Agace, Prof William
Authors: Semmrich, M., Plantinga, M., Svensson-Frej, M., Uronen-Hansson, H., Gustafsson, T., Mowat, A.M., Yrlid, U., Lambrecht, B.N., and Agace, W.W.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Mucosal Immunology
ISSN:1933-0219
ISSN (Online):1935-3456
Published Online:14 December 2011

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