Ion and solute transport by Prestin in Drosophila and Anopheles

Hirata, T., Czapar, A., Brin, L., Haritonova, A., Bondeson, D. P., Linser, P., Cabrero, P. , Thompson, J., Dow, J. A.T. and Romero, M. F. (2012) Ion and solute transport by Prestin in Drosophila and Anopheles. Journal of Insect Physiology, 58(4), pp. 563-9. (doi: 10.1016/j.jinsphys.2012.01.009) (PMID:22321763) (PMCID:PMC3482613)

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The gut and Malpighian tubules of insects are the primary sites of active solute and water transport for controlling hemolymph and urine composition, pH, and osmolarity. These processes depend on ATPase (pumps), channels and solute carriers (Slc proteins). Maturation of genomic databases enables us to identify the putative molecular players for these processes. Anion transporters of the Slc4 family, AE1 and NDAE1, have been reported as HCO(3)(-) transporters, but are only part of the story. Here we report Dipteran (Drosophila melanogaster (d) and Anopheles gambiae (Ag)) anion exchangers, belonging to the Slc26 family, which are multi-functional anion exchangers. One Drosophila and two Ag homologues of mammalian Slc26a5 (Prestin) and Slc26a6 (aka, PAT1, CFEX) were identified and designated dPrestin, AgPrestinA and AgPrestinB. dPrestin and AgPrestinB show electrogenic anion exchange (Cl(-)/nHCO(3)(-), Cl(-)/SO(4)(2-) and Cl(-)/oxalate(2-)) in an oocyte expression system. Since these transporters are the only Dipteran Slc26 proteins whose transport is similar to mammalian Slc26a6, we submit that Dipteran Prestin are functional and even molecular orthologues of mammalian Slc26a6. OSR1 kinase increases dPrestin ion transport, implying another set of physiological processes controlled by WNK/SPAK signaling in epithelia. All of these mRNAs are highly expressed in the gut and Malpighian tubules. Dipteran Prestin proteins appear suited for central roles in bicarbonate, sulfate and oxalate metabolism including generating the high pH conditions measured in the Dipteran midgut lumen. Finally, we present and discuss Drosophila genetic models that integrate these processes.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Cabrero, Mr Pablo and Dow, Professor Julian
Authors: Hirata, T., Czapar, A., Brin, L., Haritonova, A., Bondeson, D. P., Linser, P., Cabrero, P., Thompson, J., Dow, J. A.T., and Romero, M. F.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Journal of Insect Physiology
ISSN (Online):1879-1611
Published Online:30 January 2012

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
453001FlyAtlas: a comprehensive online atlas of gene expression in Drosophilia.Julian DowBiotechnology and Biological Sciences Research Council (BBSRC)BB/F010397/1Institute of Molecular Cell and Systems Biology