Comparison of visual and automated assessment of Ki-67 proliferative activity and their impact on outcome in primary operable invasive ductal breast cancer

Mohammed, Z.M.A., McMillan, D.C. , Elsberger, B., Going, J.J., Orange, C., Mallon, E., Doughty, J.C. and Edwards, J. (2012) Comparison of visual and automated assessment of Ki-67 proliferative activity and their impact on outcome in primary operable invasive ductal breast cancer. British Journal of Cancer, 106(2), pp. 383-388. (doi:10.1038/bjc.2011.569)

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Publisher's URL: http://dx.doi.org/10.1038/bjc.2011.569

Abstract

<b>BACKGROUND:</b> Immunohistochemistry of Ki-67 protein is widely used to assess tumour proliferation, and is an established prognostic factor in breast cancer. There is interest in automating the assessment of Ki-67 labelling index (LI) with possible benefits in handling increased workload, with improved accuracy and precision.<p></p> <b>PATIENTS AND METHODS:</b> Visual and automated assessment of Ki-67 LI and survival were examined in patients with primary operable invasive ductal breast cancer. Tissue microarrays (n=379 patients) immunostained for Ki-67 were scored visually and automatically with the Slidepath Tissue IA system.<p></p> <b>RESULTS:</b> Visual and automated Ki-67 LI were in excellent agreement (ICCC=0.96, P<0.001). On univariate analysis, visual (P<0.001) and automated Ki67 LI (P<0.05) were associated with cancer-specific survival in patients with invasive ductal breast cancer overall and in patients who received endocrine therapy (Tamoxifen) (P<0.01 for visual and P<0.05 for automated scoring).<p></p> <b>CONCLUSION:</b> Automated assessment of Ki-67 LI would appear to be comparable to visual Ki-67 LI. However, automated Ki-67 LI assessment was inferior in predicting cancer survival in patients with breast cancer, including patients who received Tamoxifen.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Elsberger, Miss Beatrix and McMillan, Professor Donald and Orange, Miss Clare and Mallon, Dr Elizabeth and Mohammed, Miss Zahra and Going, Dr James and Edwards, Professor Joanne and Doughty, Miss Julie
Authors: Mohammed, Z.M.A., McMillan, D.C., Elsberger, B., Going, J.J., Orange, C., Mallon, E., Doughty, J.C., and Edwards, J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Clinical Specialities
Journal Name:British Journal of Cancer
ISSN:0007-0920
ISSN (Online):1532-1827
Published Online:03 January 2012

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